CAJ | 학술논문

目的：研究致腹泻奇异变形杆菌病原学特征及毒力基因和质粒携带情况，探讨其与耐药性/致病性的关系。方法对6株不同来源（食物中毒、外环境及健康人群）的奇异变形杆菌进行生化、药敏和脉冲场凝胶电泳（PFGE）分析；PCR检测常见的毒力基因；提取质粒后电泳并切胶回收质粒进行测序分析。结果不同来源的奇异变形杆菌生化特征基本相同，且均具有常见的毒力基因ureC、rsmA、hpmA和zapA。但各菌株PFGE分型和药敏结果不同，其携带质粒情况也各异。对其中2株菌存在的小质粒测序后发现，该质粒长2683 bp，主要编码qnrD基因，与耐喹诺酮类药物相关。结论采用传统的生化分析及常见的毒力基因鉴定方法，无法区分不同来源的奇异变形杆菌，可利用PFGE和质粒分析。耐药菌株中普遍存在的质粒与菌株的耐药性相关，存在多个质粒的菌株其耐药性更强。
목적：연구치복사기이변형간균병원학특정급독력기인화질립휴대정황，탐토기여내약성/치병성적관계。방법대6주불동래원（식물중독、외배경급건강인군）적기이변형간균진행생화、약민화맥충장응효전영（PFGE）분석；PCR검측상견적독력기인；제취질립후전영병절효회수질립진행측서분석。결과불동래원적기이변형간균생화특정기본상동，차균구유상견적독력기인ureC、rsmA、hpmA화zapA。단각균주PFGE분형화약민결과불동，기휴대질립정황야각이。대기중2주균존재적소질립측서후발현，해질립장2683 bp，주요편마qnrD기인，여내규낙동류약물상관。결론채용전통적생화분석급상견적독력기인감정방법，무법구분불동래원적기이변형간균，가이용PFGE화질립분석。내약균주중보편존재적질립여균주적내약성상관，존재다개질립적균주기내약성경강。
Objective To analyze the etiological characteristics,virulence genes and plasmids that carrying diarrhea-causing Proteus mirabilis and to assess their relationship with drug resistance and pathogenicity. Methods Proteus mirabilis coming from six different sources (food poisoning,external environment and healthy people) were analyzed biochemically,on related susceptibility and pulsed-field gel electrophoresis (PFGE). Virulence genes were detected by PCR. Plasmids were extracted and sequenced after gel electrophoresis purification. Results The biochemical characteristics of Proteus mirabilis from different sources seemed basically the same,and each of them showed having common virulence genes,as ureC,rsmA,hpmA and zapA. However,the PFGE patterns and susceptibility of these strains were different,so as the plasmids that they carried. Plasmid that presented in the sequenced strain showed that the 2 683 bp length plasmid encodes qnrD gene was associated with the quinolone resistance. Conclusion Etiological characteristics and molecular characteristics of Proteus mirabilis gathered from different sources,were analyzed. Results indicated that traditional biochemical analysis and common virulence gene identification might be able to distinguish the strains with different sources. However,PFGE and plasmids analysis could distinguish the sources of strains and to identify those plasmids that commonly carried by the drug-resistant strains. These findings also provided theoretical basis for further study on the nature of resistance and pathogenicity in Proteus mirabilis.