CAJ | 학술논문

应用ELLSA试验对来自内蒙古地区17个大﹑中﹑小型奶牛场的2391份牛血清样品进行了牛病毒性腹泻/黏膜病抗体检测，并对其中222份抗体阴性牛应用ELISA试验进行牛病毒性腹泻/黏膜病的抗原检测。结果表明：17个奶牛场均检出BVDV抗体阳性，共检出阳性血清2125份，阳性率最高达100%，最低为46.8%，平均为88.9%。对14个奶牛场进行了BVDV抗原检测，在5个奶牛场检出BVDV抗原阳性，阳性率为3.6%（8/222）。表明内蒙古地区奶牛场普遍存在牛病毒性腹泻/黏膜病感染，感染率较高，并且牛群中存在持续性感染（PI）牛。
응용ELLSA시험대래자내몽고지구17개대﹑중﹑소형내우장적2391빈우혈청양품진행료우병독성복사/점막병항체검측，병대기중222빈항체음성우응용ELISA시험진행우병독성복사/점막병적항원검측。결과표명：17개내우장균검출BVDV항체양성，공검출양성혈청2125빈，양성솔최고체100%，최저위46.8%，평균위88.9%。대14개내우장진행료BVDV항원검측，재5개내우장검출BVDV항원양성，양성솔위3.6%（8/222）。표명내몽고지구내우장보편존재우병독성복사/점막병감염，감염솔교고，병차우군중존재지속성감염（PI）우。
2 391 bovine serum samples were collected from 17 dairy farms of Inner Mongolia to detect the antibody level of bovine viral diarrhea-mucosal disease by ELISA. The antigen detection of bovine viral diarrhea-mucosal disease was made on 222 antibody-negative serum samples by ELISA. The results showed that positive BVDV antibody was detected in 17 dairy farms. And there were 2 125 positive serum samples,and the highest positive rate was 100%and the lowest positive rate was 46.8%and the average positive rate was 88.9%. BVDV antigens in 14 dairy farms were detected and that in 5 dairy farms were positive. The positive rate was 3.6%(8/222). It was shown that the bovine viral diarrhea generally existed in dairy farms of Inner Mongolia with higher infection rate. And persistent infection (PI) cattle were existed in cow herd.