CAJ | 학술논문

基因组测序技术是一种分子生物学及其相关学科研究中最常用的技术。从20世纪70年代中期第一代测序技术出现以来，基因组测序技术已取得了重大进展，并改变了生命科学诸多领域的研究面貌。测序成本的急剧下降，测序通量呈指数提高，使得测序速度大大提升。DNA和RNA是生命体的2个基本遗传物质，其组成和序列变化创造了形形色色的生命世界。快速、准确地获取生物体的遗传信息对于生命科学的研究具有重要意义，测序技术能够真正地反映基因组、遗传信息转录，全面地揭示基因组的复杂性和多样性，在生命科学研究中起着重要的作用。综述了各代测序技术的发展成果、测序原理、测序优缺点、国内研究现状，并对测序技术未来的发展方向进行了展望。
기인조측서기술시일충분자생물학급기상관학과연구중최상용적기술。종20세기70년대중기제일대측서기술출현이래，기인조측서기술이취득료중대진전，병개변료생명과학제다영역적연구면모。측서성본적급극하강，측서통량정지수제고，사득측서속도대대제승。DNA화RNA시생명체적2개기본유전물질，기조성화서렬변화창조료형형색색적생명세계。쾌속、준학지획취생물체적유전신식대우생명과학적연구구유중요의의，측서기술능구진정지반영기인조、유전신식전록，전면지게시기인조적복잡성화다양성，재생명과학연구중기착중요적작용。종술료각대측서기술적발전성과、측서원리、측서우결점、국내연구현상，병대측서기술미래적발전방향진행료전망。
Genome sequencing technology is the most commonly used technique in molecular biology and its related discipline studies. Genome sequencing technology has made great progresses and changed the status of life science research in many fields since the first-generation of sequencing technology appeared in the 1970s. The sharp decline of sequencing cost and exponential increase of sequencing throughout greatly improved the sequencing speed. The two basic genetic materials of organism are DNA and RNA, whose composition and sequence changes create all sorts of lives in the world. It is of important significance to get the genetic information of organisms fast and accurately for life science research. Sequencing technology can reflect genome and transcription of genetic information truly and reveal the complexity and diversity of genome, which plays a very important role in life science research. The development achievements, sequencing principle, the advantages and disadvantages of sequencing, domestic research status of each generation of sequencing technology were reviewed. And the future development direction of sequencing technology was predicted.