CAJ | 학술논문

目的：研究P-糖蛋白（P-gp）表达与原发性肝细胞癌合并门静脉癌栓患者表达情况与临床化疗效果之间的关系。方法收集术后接受化疗的29例原发性肝细胞癌合并门静脉癌栓患者的石蜡块，构建组织芯片。采用免疫组化染色技术，检测P-gp蛋白的表达情况。根据P-gp蛋白表达强弱分为耐药组和非耐药组，进行生存分析。结果P-gp蛋白表达的阳性率为75.86％（22/29）。中位无瘤生存期在耐药组（20例）与非耐药组（9例）间有显著差别。多因素Cox回归分析显示P-gp表达情况为无瘤生存期的独立影响因子。结论P-gp的强表达是导致肝癌耐药、化疗效果欠佳的原因之一，P-gp有可能作为临床筛选化疗患者的指标。
목적：연구P-당단백（P-gp）표체여원발성간세포암합병문정맥암전환자표체정황여림상화료효과지간적관계。방법수집술후접수화료적29례원발성간세포암합병문정맥암전환자적석사괴，구건조직심편。채용면역조화염색기술，검측P-gp단백적표체정황。근거P-gp단백표체강약분위내약조화비내약조，진행생존분석。결과P-gp단백표체적양성솔위75.86％（22/29）。중위무류생존기재내약조（20례）여비내약조（9례）간유현저차별。다인소Cox회귀분석현시P-gp표체정황위무류생존기적독립영향인자。결론P-gp적강표체시도치간암내약、화료효과흠가적원인지일，P-gp유가능작위림상사선화료환자적지표。
Objective To investigate the expression of P-glycoprotein (P-gp) and its correlation to chemotherapeutic efficacy in hepatocellular carcinoma (HCC) with tumor thrombi in major portal vein. Methods Twenty-nine patients of HCC with tumor thrombi in major portal vein following hepatectomy and thrombectomy were enrolled in this study, all the patients received immunochemotherapy postperatively. Specimens embedded in paraffin were collected for the construction of tissue microarrays. P-gp protein were visualized by immunohistochemical staining using Monoclonal antibodies JSB-1. The patients were divided into chemotherapy sensitive and resistant groups according to the staining grade. The intensity≥(++) were considered resistant to chemotherapy. Survival time was compared between the two groups and prognostic factors were identified using Cox proportional hazards model. Results The positive rate of P-gp was 75.86% (22/29). Medium tumor-free survival time showed significant differences between two groups. Multivariate analysis revealed that the expression grade of P-gp was independent prognostic factor for tumor-free survival time. Conclusion In HCC with tumor thrombi in major portal vein, the overexpression of P-gp is one of the major reason for resistance to chemotherapy, detection of which will be helpful in chemotherapy decision making.