食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2013年
5期
1543-1549
,共7页
蛋清蛋白肽%超滤%抗氧化作用
蛋清蛋白肽%超濾%抗氧化作用
단청단백태%초려%항양화작용
egg white-derived peptides%ultrafiltration%antioxidant
目的：研究蛋清蛋白肽抗氧化作用模式。方法利用超滤技术分离蛋清蛋白木瓜蛋白酶酶解产物；采用 Fenton 体系、邻苯三酚自氧化体系和亚油酸自氧化体系分别测定超滤各组分清除羟自由基、超氧阴离子及抑制脂质过氧化的能力,同时测定各组分对二苯代苦味肼基自由基清除能力(DPPH 自由基)、还原能力及对猪胎儿成纤维细胞(porcine embryonic fibroblast, PEF)过氧化损伤的保护作用。结果超滤各组分中分子量小于3 kDa组分(蛋清蛋白酶解产物-Ⅲ, egg white protein hydrolysate, EWPH-Ⅲ)占蛋清蛋白酶解产物(EWPHs)总量的50.06%。EWPH的抗氧化活性随分子量的降低而增强(P<0.05),其中EWPH-Ⅲ在浓度为5 mg/mL时,对羟自由基、超氧阴离子、DPPH自由基的清除率分别为52.86%、35.05%和78.74%,对亚油酸氧化的抑制率为74.57%。在浓度为2.5 mg/mL时, PEF细胞存活率达到70.06%。结论蛋清蛋白肽具有较强的抗氧化活性且分子量越小,抗氧化活性越强,可以作为氢供体、自由基稳定剂和金属离子螯合剂来抑制过氧化作用。
目的：研究蛋清蛋白肽抗氧化作用模式。方法利用超濾技術分離蛋清蛋白木瓜蛋白酶酶解產物；採用 Fenton 體繫、鄰苯三酚自氧化體繫和亞油痠自氧化體繫分彆測定超濾各組分清除羥自由基、超氧陰離子及抑製脂質過氧化的能力,同時測定各組分對二苯代苦味肼基自由基清除能力(DPPH 自由基)、還原能力及對豬胎兒成纖維細胞(porcine embryonic fibroblast, PEF)過氧化損傷的保護作用。結果超濾各組分中分子量小于3 kDa組分(蛋清蛋白酶解產物-Ⅲ, egg white protein hydrolysate, EWPH-Ⅲ)佔蛋清蛋白酶解產物(EWPHs)總量的50.06%。EWPH的抗氧化活性隨分子量的降低而增彊(P<0.05),其中EWPH-Ⅲ在濃度為5 mg/mL時,對羥自由基、超氧陰離子、DPPH自由基的清除率分彆為52.86%、35.05%和78.74%,對亞油痠氧化的抑製率為74.57%。在濃度為2.5 mg/mL時, PEF細胞存活率達到70.06%。結論蛋清蛋白肽具有較彊的抗氧化活性且分子量越小,抗氧化活性越彊,可以作為氫供體、自由基穩定劑和金屬離子螯閤劑來抑製過氧化作用。
목적：연구단청단백태항양화작용모식。방법이용초려기술분리단청단백목과단백매매해산물；채용 Fenton 체계、린분삼분자양화체계화아유산자양화체계분별측정초려각조분청제간자유기、초양음리자급억제지질과양화적능력,동시측정각조분대이분대고미정기자유기청제능력(DPPH 자유기)、환원능력급대저태인성섬유세포(porcine embryonic fibroblast, PEF)과양화손상적보호작용。결과초려각조분중분자량소우3 kDa조분(단청단백매해산물-Ⅲ, egg white protein hydrolysate, EWPH-Ⅲ)점단청단백매해산물(EWPHs)총량적50.06%。EWPH적항양화활성수분자량적강저이증강(P<0.05),기중EWPH-Ⅲ재농도위5 mg/mL시,대간자유기、초양음리자、DPPH자유기적청제솔분별위52.86%、35.05%화78.74%,대아유산양화적억제솔위74.57%。재농도위2.5 mg/mL시, PEF세포존활솔체도70.06%。결론단청단백태구유교강적항양화활성차분자량월소,항양화활성월강,가이작위경공체、자유기은정제화금속리자오합제래억제과양화작용。
Objective To investigate antioxidantive action mode of egg white-derived peptides in vitro. Methods The egg white-derived peptides were separated by ultrafiltration from egg white protein hydroly-sates (EWPHs) produced by papain into three fractions. The hydroxyl radical, superoxide anion scavenging ac-tivities and lipid peroxidation inhibition of three fractions were detected by the methods related to Fenton sys-tem, pyrogallol autoxidation, and linoleic acid emulsion system. The reducing power, DPPH radical scavenging activities and alleviation of H2O2-induced oxidative stress on porcine embryonic fibroblast (PEF) cell were also determined. Results EWPH-Ⅲ (MW<3 kDa) accounted for 50.06% of the total amount of EWPHs. The values of hydroxyl radical, superoxide anion and DPPH radical scavenging activities were 52.86%, 35.05%and 78.74% at the concentration of 5 mg/mL, respectively. The percentage of inhibition of lipid peroxidation was 74.57% at the same concentration. The cell survival rate reached 70.06% at the concentration of 2.5mg/mL. EWPH exhibited significant increase (P<0.05) in antioxidant activity with a decrease in molecular weight of fraction values. Conclusion EWPH could act as a metal ion chelator, a hydrogen donor, as well as a radical stabilizer to inhibit lipid oxidation because of its antioxidant activity.
