CAJ | 학술논문

【目的】优化水解醇溶蛋白生产功能肽的工艺，提高玉米醇溶蛋白的应用价值．【方法】通过正交试验获得从玉米蛋白粉中提取醇溶蛋白的最佳提取时间、提取温度、料液比、乙醇浓度等提取条件；通过碱性蛋白酶和蛋白酶K对玉米醇溶蛋白进行水解生产玉米多肽．【结果和结论】通过正交试验获得了从玉米蛋白粉中提取醇溶蛋白的最佳条件，即：料液比1∶4（g/mL），60℃水浴加热，乙醇体积分数为75％，提取3 h．两步酶解法效率高于单酶解法，两步酶解法先加蛋白酶K反应后再加碱性蛋白酶时水解效果最佳．
【목적】우화수해순용단백생산공능태적공예，제고옥미순용단백적응용개치．【방법】통과정교시험획득종옥미단백분중제취순용단백적최가제취시간、제취온도、료액비、을순농도등제취조건；통과감성단백매화단백매K대옥미순용단백진행수해생산옥미다태．【결과화결론】통과정교시험획득료종옥미단백분중제취순용단백적최가조건，즉：료액비1∶4（g/mL），60℃수욕가열，을순체적분수위75％，제취3 h．량보매해법효솔고우단매해법，량보매해법선가단백매K반응후재가감성단백매시수해효과최가．
[Objective]The production of short peptide from zein was investigated to greatly promote the application of zein .[Method]The conditions such as extraction time , temperature , ethanol concentration and ratio of liquid to solid were optimized by orthogonal test .Zein was hydrolyzed by protease K and al-kaline protease in this study .[Result and conclusion]The optimal conditions of extracting zein were as follows:the ratio of solid to liquid was 1∶4;the extracting time was 3 hours;the extracting temperature was 60 ℃and the concentration of ethanol was 75%.Results showed that the zein could be hydrolyzed more efficiently by combining two enzymes than by only using one of the two enzymes .Zein hydrolyzed by protease K and alkaline protease in turn is the best enzymatic hydrolysis method .