华南农业大学学报
華南農業大學學報
화남농업대학학보
JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY
2014年
3期
74-78
,共5页
王蕾%陈玉婷%蔡昆争%汪国平
王蕾%陳玉婷%蔡昆爭%汪國平
왕뢰%진옥정%채곤쟁%왕국평
硅%番茄叶片%青枯病%抗病性%抗氧化酶活性
硅%番茄葉片%青枯病%抗病性%抗氧化酶活性
규%번가협편%청고병%항병성%항양화매활성
silicon%tomato leaves%bacterial wilt%disease resistance%antioxidant enzyme activity
【目的】探讨青枯菌侵染下外源硅处理对番茄青枯病的抗性效果及生理作用机理,为青枯病的有效防治提供参考依据.【方法】选择青枯病易感番茄品种为试验材料,通过盆栽土培和水培试验,研究硅处理和青枯菌Ralstonia solanacearum接种对番茄青枯病病情指数、叶片抗氧化酶活性的影响.【结果和结论】硅能显著增强番茄青枯病抗性.硅处理使青枯病的病情指数在土培试验和水培试验下分别降低29.1%~93.0%和6.3%~100.0%.青枯菌侵染条件下硅处理能显著增加番茄叶片抗氧化酶活性.在土培试验中,加硅使番茄叶片的POD、CAT酶活性分别增加了43.17%和23.17%;水培试验在接种第3天,加硅使POD、CAT、PAL酶活性分别增加了122%、337%和31%.本研究表明硅对番茄青枯病的抗性机理可能是硅诱导植物产生一系列生物化学防御反应,增强了番茄对青枯病菌的抗性.
【目的】探討青枯菌侵染下外源硅處理對番茄青枯病的抗性效果及生理作用機理,為青枯病的有效防治提供參攷依據.【方法】選擇青枯病易感番茄品種為試驗材料,通過盆栽土培和水培試驗,研究硅處理和青枯菌Ralstonia solanacearum接種對番茄青枯病病情指數、葉片抗氧化酶活性的影響.【結果和結論】硅能顯著增彊番茄青枯病抗性.硅處理使青枯病的病情指數在土培試驗和水培試驗下分彆降低29.1%~93.0%和6.3%~100.0%.青枯菌侵染條件下硅處理能顯著增加番茄葉片抗氧化酶活性.在土培試驗中,加硅使番茄葉片的POD、CAT酶活性分彆增加瞭43.17%和23.17%;水培試驗在接種第3天,加硅使POD、CAT、PAL酶活性分彆增加瞭122%、337%和31%.本研究錶明硅對番茄青枯病的抗性機理可能是硅誘導植物產生一繫列生物化學防禦反應,增彊瞭番茄對青枯病菌的抗性.
【목적】탐토청고균침염하외원규처리대번가청고병적항성효과급생리작용궤리,위청고병적유효방치제공삼고의거.【방법】선택청고병역감번가품충위시험재료,통과분재토배화수배시험,연구규처리화청고균Ralstonia solanacearum접충대번가청고병병정지수、협편항양화매활성적영향.【결과화결론】규능현저증강번가청고병항성.규처리사청고병적병정지수재토배시험화수배시험하분별강저29.1%~93.0%화6.3%~100.0%.청고균침염조건하규처리능현저증가번가협편항양화매활성.재토배시험중,가규사번가협편적POD、CAT매활성분별증가료43.17%화23.17%;수배시험재접충제3천,가규사POD、CAT、PAL매활성분별증가료122%、337%화31%.본연구표명규대번가청고병적항성궤리가능시규유도식물산생일계렬생물화학방어반응,증강료번가대청고병균적항성.
[Objective]The objective of this study was to investigate the physiological mechanism of sili-con-induced resistance of tomato plant to bacterial wilt .[Method]Pot experiments with soil and hydropo-nic cultivation were conducted to study the effects of silicon supply on the disease index of bacterial wilt and antioxidant enzymes of tomato leaves .[Result and conclusion]The results showed that exogenous Si treatment reduced the disease index of bacterial wilt by 29.1%-93.0% respectively in soil cultivation experiment and 6.3% -100% respectively in hydroponic experiment compared with no-Si-treatment.Compared with the no-silicon treatment , Si application increased POD and CAT activity of tomato leaves by 43.17% and 23.17% in soil cultivation experiment .Si amendments significantly increased POD , CAT and PAL activity of tomato leaves by 122%, 337% and 31% respectively in hydroponic experi-ments.In conclusion, Si supply has an important role in suppressing bacterial wilt caused by Ralstonia solanacearum.The pathogen resistance mediated by Si is associated with the activation of defense-related enzyme activities in leaves .