浙江临床医学
浙江臨床醫學
절강림상의학
ZHEJIANG CLINICAL MEDICAL JOURNAL
2014年
4期
512-515
,共4页
陈孝天%彭三妹%王博林%陈如意%袁小凤%林瑶
陳孝天%彭三妹%王博林%陳如意%袁小鳳%林瑤
진효천%팽삼매%왕박림%진여의%원소봉%림요
溃疡性结肠炎%微生物多样性%聚合酶链反应-变性梯度凝胶电泳(PCR-DGGE)%2,4-二硝基氯苯乙酸复合法
潰瘍性結腸炎%微生物多樣性%聚閤酶鏈反應-變性梯度凝膠電泳(PCR-DGGE)%2,4-二硝基氯苯乙痠複閤法
궤양성결장염%미생물다양성%취합매련반응-변성제도응효전영(PCR-DGGE)%2,4-이초기록분을산복합법
Ulcerative colitis%Microbial diversity%PCR-DGGE%2,4-Dinitrochlorobenzene plus acetic acid compound method
目的：探讨溃疡性结肠炎对大鼠胃肠道微生物多样性影响，为治疗溃疡性结肠炎提供可靠依据。方法采用2，4-二硝基氯苯乙酸复合法对健康SD大鼠进行溃疡性结肠炎造模；运用CTAB-SDS法提取肠道微生物总DNA，通过PCR-DGGE分析溃疡性结肠炎大鼠之间胃肠道微生物多样性差异。结果溃疡组、用药组和正常组大鼠的消化道中，不同部位细菌Shannon指数变化较大（1.64~3.05），结肠中Shannon指数最高，其次是胃，小肠最低。在结肠，不同组的细菌多样性Shannon指数变化幅度不大，以用药组最高（3.05），其次溃疡组（2.98），正常组2.95，说明大鼠结肠在健康状态下细菌多样性最低，结肠炎后会发生一定的变化。此外，发现病变后的大鼠胃中拟杆菌属、梭菌属、普氏菌属、紫单胞菌属等厌氧细菌增加，病变后的结肠中乳杆菌属细菌明显减少，紫单胞菌属和梭菌属细菌明显增加；病变后小肠中部分乳杆菌种类消失，同时梭菌属和紫单胞菌属等不常见菌群的出现。结论溃疡性结肠炎大鼠胃、小肠、结肠中有益菌乳杆菌明显减少，普氏菌属、紫单胞菌属、梭菌属等明显增多，这些菌属可作为条件致病菌引起内源性感染，可能是造成溃疡性结肠炎的重要原因。
目的：探討潰瘍性結腸炎對大鼠胃腸道微生物多樣性影響，為治療潰瘍性結腸炎提供可靠依據。方法採用2，4-二硝基氯苯乙痠複閤法對健康SD大鼠進行潰瘍性結腸炎造模；運用CTAB-SDS法提取腸道微生物總DNA，通過PCR-DGGE分析潰瘍性結腸炎大鼠之間胃腸道微生物多樣性差異。結果潰瘍組、用藥組和正常組大鼠的消化道中，不同部位細菌Shannon指數變化較大（1.64~3.05），結腸中Shannon指數最高，其次是胃，小腸最低。在結腸，不同組的細菌多樣性Shannon指數變化幅度不大，以用藥組最高（3.05），其次潰瘍組（2.98），正常組2.95，說明大鼠結腸在健康狀態下細菌多樣性最低，結腸炎後會髮生一定的變化。此外，髮現病變後的大鼠胃中擬桿菌屬、梭菌屬、普氏菌屬、紫單胞菌屬等厭氧細菌增加，病變後的結腸中乳桿菌屬細菌明顯減少，紫單胞菌屬和梭菌屬細菌明顯增加；病變後小腸中部分乳桿菌種類消失，同時梭菌屬和紫單胞菌屬等不常見菌群的齣現。結論潰瘍性結腸炎大鼠胃、小腸、結腸中有益菌乳桿菌明顯減少，普氏菌屬、紫單胞菌屬、梭菌屬等明顯增多，這些菌屬可作為條件緻病菌引起內源性感染，可能是造成潰瘍性結腸炎的重要原因。
목적：탐토궤양성결장염대대서위장도미생물다양성영향，위치료궤양성결장염제공가고의거。방법채용2，4-이초기록분을산복합법대건강SD대서진행궤양성결장염조모；운용CTAB-SDS법제취장도미생물총DNA，통과PCR-DGGE분석궤양성결장염대서지간위장도미생물다양성차이。결과궤양조、용약조화정상조대서적소화도중，불동부위세균Shannon지수변화교대（1.64~3.05），결장중Shannon지수최고，기차시위，소장최저。재결장，불동조적세균다양성Shannon지수변화폭도불대，이용약조최고（3.05），기차궤양조（2.98），정상조2.95，설명대서결장재건강상태하세균다양성최저，결장염후회발생일정적변화。차외，발현병변후적대서위중의간균속、사균속、보씨균속、자단포균속등염양세균증가，병변후적결장중유간균속세균명현감소，자단포균속화사균속세균명현증가；병변후소장중부분유간균충류소실，동시사균속화자단포균속등불상견균군적출현。결론궤양성결장염대서위、소장、결장중유익균유간균명현감소，보씨균속、자단포균속、사균속등명현증다，저사균속가작위조건치병균인기내원성감염，가능시조성궤양성결장염적중요원인。
Objective The effects of ulcerative colitis(UC)on microbial diversity in the rat gastrointestinal tract were studied to provide a reliable basis for treating ulcerative colitis. Method Rat UC models were set up with 2,4-Dinitrochlorobenzene plus acetic acid compound method. The total microbial DNA was extracted from intestine with CTAB-SDS method. Analyzing microbial diversity in the rats gastrointestinal tract by denaturing gradient gel electrophoresis(DGGE). Results Comparing the results among these three groups(model group,medicated group,normal control group),we found that the Shannon index of bacteria varied from 1.64 to 3.05 among different parts of digestive tract. The highest one was in colon,then was in stomach and the lowest one was in small intestine . As for Shannon index of bacteria in colon,the medical group with 3.05 was the highest,followed by model group with 2.98,normal control group with 2.95,which showed that the microbial diversity in colon changed by the colitis. From the sequence analysis results we found that the numbers of bacteroides,clostridium,prevotella,porphyromonas in stomach of rat increased after getting ulcerative colitis,while in colon the number of lactobacillus decreased and clostridium,porphyromonas increased. Besides,in small intestine some species of lactobacillus disappeared and some uncommon flora such as clostridium and Porphyromonas appeared. Conclusion In stomach,small intestine and colon of UC rat,the number of beneficial bacteria lactobacillus significantly decreases while clostridium,prevotella and porphyromonas increase,which may finally cause endogenous infection and be an important reason for ulcerative colitis.
