中国妇幼健康研究
中國婦幼健康研究
중국부유건강연구
CHINESE JOURNAL OF MATERNAL AND CHILD HEALTH RESEARCH
2014年
2期
273-275
,共3页
卵巢肿瘤%人附睾上皮分泌蛋白4%糖链抗原CA125%灵敏度%特异度
卵巢腫瘤%人附睪上皮分泌蛋白4%糖鏈抗原CA125%靈敏度%特異度
란소종류%인부고상피분비단백4%당련항원CA125%령민도%특이도
ovarian tumor%human epididymis protein 4 (HE4)%carbohydrate antigen125 (CA125)%sensitivity%specificity
目的：探讨人附睾上皮分泌蛋白4（HE4）对卵巢癌的诊断意义及其与糖链抗原CA125联合检测对卵巢良、恶性肿瘤的诊断价值。方法检测22例卵巢癌患者、46例卵巢良性肿瘤患者及35例健康女性血清中的HE4和CA125水平。 CA125的测定采用适用于96微孔反应板的时间分辨荧光测定仪测量，通过双抗体夹心时间分辨免疫荧光分析法进行测定。 HE4的测定应用电化学发光法进行测定。结果卵巢癌组HE4水平明显高于卵巢良性肿瘤和健康对照组，差异均具有统计学意义（t1＝3．356， t2＝3．865，均P＜0．05）；卵巢癌组血清CA125水平也高于卵巢良性肿瘤组和健康对照组，差异均有统计学意义（ t1＝4．656，t2＝5．126，均P＜0．05）。单独检测HE4的灵敏度和特异度分别为77．27％和84．78％；单独检测CA125的灵敏度和特异度分别为90．91％和80．43％；二者联合检测时灵敏度和特异度分别为72．73％和89．13％。结论 HE4对卵巢癌的诊断有较高的特异度；CA125的特异度低于HE4；HE4对卵巢癌的诊断价值优于单独CA125检测；联合检测提高了特异度，降低了的灵敏度。
目的：探討人附睪上皮分泌蛋白4（HE4）對卵巢癌的診斷意義及其與糖鏈抗原CA125聯閤檢測對卵巢良、噁性腫瘤的診斷價值。方法檢測22例卵巢癌患者、46例卵巢良性腫瘤患者及35例健康女性血清中的HE4和CA125水平。 CA125的測定採用適用于96微孔反應闆的時間分辨熒光測定儀測量，通過雙抗體夾心時間分辨免疫熒光分析法進行測定。 HE4的測定應用電化學髮光法進行測定。結果卵巢癌組HE4水平明顯高于卵巢良性腫瘤和健康對照組，差異均具有統計學意義（t1＝3．356， t2＝3．865，均P＜0．05）；卵巢癌組血清CA125水平也高于卵巢良性腫瘤組和健康對照組，差異均有統計學意義（ t1＝4．656，t2＝5．126，均P＜0．05）。單獨檢測HE4的靈敏度和特異度分彆為77．27％和84．78％；單獨檢測CA125的靈敏度和特異度分彆為90．91％和80．43％；二者聯閤檢測時靈敏度和特異度分彆為72．73％和89．13％。結論 HE4對卵巢癌的診斷有較高的特異度；CA125的特異度低于HE4；HE4對卵巢癌的診斷價值優于單獨CA125檢測；聯閤檢測提高瞭特異度，降低瞭的靈敏度。
목적：탐토인부고상피분비단백4（HE4）대란소암적진단의의급기여당련항원CA125연합검측대란소량、악성종류적진단개치。방법검측22례란소암환자、46례란소량성종류환자급35례건강녀성혈청중적HE4화CA125수평。 CA125적측정채용괄용우96미공반응판적시간분변형광측정의측량，통과쌍항체협심시간분변면역형광분석법진행측정。 HE4적측정응용전화학발광법진행측정。결과란소암조HE4수평명현고우란소량성종류화건강대조조，차이균구유통계학의의（t1＝3．356， t2＝3．865，균P＜0．05）；란소암조혈청CA125수평야고우란소량성종류조화건강대조조，차이균유통계학의의（ t1＝4．656，t2＝5．126，균P＜0．05）。단독검측HE4적령민도화특이도분별위77．27％화84．78％；단독검측CA125적령민도화특이도분별위90．91％화80．43％；이자연합검측시령민도화특이도분별위72．73％화89．13％。결론 HE4대란소암적진단유교고적특이도；CA125적특이도저우HE4；HE4대란소암적진단개치우우단독CA125검측；연합검측제고료특이도，강저료적령민도。
Objective To explore the diagnostic value of human epididymis protein 4 (HE4) in ovarian cancer and that of HE4 combined with carbohydrate antigen 125 ( CA125) in human ovarian cancer and ovarian benign tumors .Methods Serum levels of HE4 and CA125 in 22 cases of ovarian cancer , 46 cases of ovarian benign tumors and 35 cases of healthy controls were detected .Time-resolved fluorescence measurement apparatus applicable to 96 microporous reaction plate was used , and CA125 was measured by double antibody sandwich time-resolved immunofluorescence analysis method .Electrochemical luminescence method was used to determine HE 4.Results Serum levels of HE4 among ovarian cancer patients were obviously higher than those of ovarian benign tumors and healthy controls , and the difference was statistically significant (t1 =3.356,t2 =3.865, both P<0.05).Serum levels of CA125 among ovarian cancer patients were obviously higher than those of ovarian benign tumors and healthy controls , and there was significant difference ( t1 =4.656,t2 =5.126, both P<0.05).The sensitivity and specificity of HE4 was 77.27%and 84.78%, respectively, while the sensitivity and specificity of CA125 was 90.91%and 80.43%, respectively.The sensitivity and specificity of joint detection was 72.73%and 89.13%, respectively.Conclusion HE4 has high specificity in detecting ovarian cancer , which is higher than CA125.The diagnostic value of HE4 is superior to CA125 in detecting ovarian cancer .Joint detection improves specificity but reduces sensitivity .
