徐州医学院学报
徐州醫學院學報
서주의학원학보
ACTA ACADEMIAE MEDICINAE XUZHOU
2014年
2期
99-102
,共4页
Balb/c小鼠%地塞米松%卡氏肺孢子虫肺炎%动物模型%Giemsa染色
Balb/c小鼠%地塞米鬆%卡氏肺孢子蟲肺炎%動物模型%Giemsa染色
Balb/c소서%지새미송%잡씨폐포자충폐염%동물모형%Giemsa염색
Balb/c mice%dexamethasone%Pneumocystis cariniipneumonia%animal model%Giemsa staining
目的：比较皮下注射和口服地塞米松建立Balb/c小鼠卡氏肺孢子虫肺炎（ PCP）模型的差异，探索2种方法的优劣。方法将雌性Balb/c小鼠随机分为地塞米松皮下注射组（注射组）、地塞米松口服组（口服组）和对照组。注射组采用每3天皮下注射地塞米松1次（0．5 mg/次，共16次）的方法诱发PCP；口服组经口灌服1 mg/L的地塞米松溶液1 ml，持续6周；对照1组注射等体积生理盐水；对照2组不给任何药物。结果注射组无死亡小鼠，51天后卡氏肺孢子虫（PC）总感染率为90％（27/30）；口服组2只小鼠死亡，63天后PC总感染率为67％（20/30）；2组比较差异有统计学意义（P＜0．05）。对照组未检出PC包囊。结论皮下注射和口服地塞米松方法均可成功建立Balb/c小鼠PCP动物模型。皮下注射方法与口服方法比较，具有感染率高、死亡率低、诱导时间短的优点。
目的：比較皮下註射和口服地塞米鬆建立Balb/c小鼠卡氏肺孢子蟲肺炎（ PCP）模型的差異，探索2種方法的優劣。方法將雌性Balb/c小鼠隨機分為地塞米鬆皮下註射組（註射組）、地塞米鬆口服組（口服組）和對照組。註射組採用每3天皮下註射地塞米鬆1次（0．5 mg/次，共16次）的方法誘髮PCP；口服組經口灌服1 mg/L的地塞米鬆溶液1 ml，持續6週；對照1組註射等體積生理鹽水；對照2組不給任何藥物。結果註射組無死亡小鼠，51天後卡氏肺孢子蟲（PC）總感染率為90％（27/30）；口服組2隻小鼠死亡，63天後PC總感染率為67％（20/30）；2組比較差異有統計學意義（P＜0．05）。對照組未檢齣PC包囊。結論皮下註射和口服地塞米鬆方法均可成功建立Balb/c小鼠PCP動物模型。皮下註射方法與口服方法比較，具有感染率高、死亡率低、誘導時間短的優點。
목적：비교피하주사화구복지새미송건립Balb/c소서잡씨폐포자충폐염（ PCP）모형적차이，탐색2충방법적우렬。방법장자성Balb/c소서수궤분위지새미송피하주사조（주사조）、지새미송구복조（구복조）화대조조。주사조채용매3천피하주사지새미송1차（0．5 mg/차，공16차）적방법유발PCP；구복조경구관복1 mg/L적지새미송용액1 ml，지속6주；대조1조주사등체적생리염수；대조2조불급임하약물。결과주사조무사망소서，51천후잡씨폐포자충（PC）총감염솔위90％（27/30）；구복조2지소서사망，63천후PC총감염솔위67％（20/30）；2조비교차이유통계학의의（P＜0．05）。대조조미검출PC포낭。결론피하주사화구복지새미송방법균가성공건립Balb/c소서PCP동물모형。피하주사방법여구복방법비교，구유감염솔고、사망솔저、유도시간단적우점。
Objective To compare the difference between two Pneumocystis carinii pneumonia ( PCP ) models of Balb/c mice induced by dexamethasone through subcutaneous injection and oral administration .Methods Female Balb/c mice were randomly divided into a subcutaneous injection of dexamethasone group (Injection), an oral dexamethasone group ( Oral) and control groups .The Injection group was subcutaneously injected with dexamethasone once every three days (0.5 mg/per time, 16 times in total) to induce PCP.The oral group was orally taken 1 ml of 1 mg/L dexametha-sone for consecutive 6 weeks.The mice in the control group 1 were injected with an equal volume of normal saline , while those in the control group 2 did not receive any medication .Results In the Injection group , no dead mice were reported and fifty-one days later, the total infection rate of Pneumocystis carinii was 90%(27/30).In the Oral group, two dead mice were found and sixty -three days later the total infection rat was 67% ( 20/30 ) .The differences were statistically significant (P<0.05).No Pneumocystis carinii was detected in control groups .Conclusion The PCP model of Balb/c mice can be successfully established through induction of dexamethasone through both subcutaneous injection and oral ad -ministration.Compared with oral route , subcutaneous injection can cause a high infection rate , a low mortality rate and short induction duration .
