CAJ | 학술논문

目的：了解福建省HIV-1新近感染者流行毒株的亚型分布和流行特征。方法随机抽取福建省2011-2012年使用BED捕获酶免疫试验检出的104例新近感染者血样标本，采用反转录巢式PCR扩增gag、pol和env基因片段，并测定和分析核酸序列。结果目标人群中存在B亚型和01_AE、07_BC、08_BC、55_01B四种流行重组型，其中以01_AE和07_BC流行重组型为主，分别占45.68%和35.80%。基因亚型的流行特征分析显示，亚型分布中01_AE所占比例下降，07_BC所占比例上升；性传播是主要感染途径，其中同性传播比例明显上升。结论福建省HIV-1流行毒株亚型分布众多，随着时间推移，亚型构成和传播途径已发生改变，应加强对HIV-1感染的分子流行病学趋势动态监测。
목적：료해복건성HIV-1신근감염자류행독주적아형분포화류행특정。방법수궤추취복건성2011-2012년사용BED포획매면역시험검출적104례신근감염자혈양표본，채용반전록소식PCR확증gag、pol화env기인편단，병측정화분석핵산서렬。결과목표인군중존재B아형화01_AE、07_BC、08_BC、55_01B사충류행중조형，기중이01_AE화07_BC류행중조형위주，분별점45.68%화35.80%。기인아형적류행특정분석현시，아형분포중01_AE소점비례하강，07_BC소점비례상승；성전파시주요감염도경，기중동성전파비례명현상승。결론복건성HIV-1류행독주아형분포음다，수착시간추이，아형구성화전파도경이발생개변，응가강대HIV-1감염적분자류행병학추세동태감측。
Objective In order to evaluate the distribution of genetic subtypes and epidemiological feature of HIV-1 circulating strains in Fujian province. Methods Blood samples and epidemiological data were collected from 104 newly infected patients who were distinguished by BED-CEIA methodology,during 2011-2012. Viral sequences (n=81) of HIV-1 gag,env,and pol segments were amplified by nested PCR. Results Subtypes B and four Circulating Recombinant Forms,(CRF01_AE,CRF07_BC,CRF08_BC and CRF55_01B) were found in the samples, CRF01_AE(45.68%)and CRF07_BC(35.80%)were the two main HIV-1 strains in Fujian province. Compared with previous data,the proportion of CRF07_BC rose significantly while it gradually decreased in CRF01_AE. Heterosexual contact was still the principal transmission route in Fujian province, but the number of infection among men-who-have-sex-with- men grew rapidly. Conclusion Results from this study suggested that different subtypes of HIV-1 strain existed in Fujian province. The distribution of subtypes and the mode of transmission were changing with the progress of epidemic. Dynamic monitoring of the molecular epidemiology trends of HIV-1 infection should be enhanced.