实用医学杂志
實用醫學雜誌
실용의학잡지
THE JOURNAL OF PRACTICAL MEDICINE
2014年
4期
525-527,528
,共4页
支气管肺发育不良%高氧%血管内皮生长因子%血管生成素
支氣管肺髮育不良%高氧%血管內皮生長因子%血管生成素
지기관폐발육불량%고양%혈관내피생장인자%혈관생성소
BPD%Hyperoxia%VEGF%Ang
目的：研究高氧诱导新生鼠急性肺损伤 VEGF 和 Ang-1的表达及其对肺发育的影响，为临床防治支气管肺发育不良（BPD）提供实验依据。方法：2～3日龄新生SD大鼠共48只随机分组，于空气及高氧中喂养，采用实时荧光定量PCR和蛋白质免疫印迹方法检测第1、3、7天肺组织VEGF、Ang-1mRNA 和蛋白表达水平，观察肺组织形态结构的变化。结果：第7天VEGF、Ang-1 mRNA 相对含量，对照组为0．722±0．372、0．828±0．462，高氧组为0．239±0．293、0．327±0．184；VEGF、Ang-1蛋白相对含量，对照组为0．632±0．289、0．573±0．436，高氧组为0．358±0．128、0．204±0．068。第7天VEGF、Ang-1 mRNA 和蛋白表达水平在对照组和高氧组比较差异均存在显著性（P＜0．05）。光镜下观察高氧组肺组织表现为肺组织显著发育不良，肺泡结构简单化，肺泡数目减少和肺微血管发育受阻。结论：VEGF和Ang-1是肺血管发育过程中不可或缺的调节因子，参与了BPD病理发生，对肺发育起着重要作用。
目的：研究高氧誘導新生鼠急性肺損傷 VEGF 和 Ang-1的錶達及其對肺髮育的影響，為臨床防治支氣管肺髮育不良（BPD）提供實驗依據。方法：2～3日齡新生SD大鼠共48隻隨機分組，于空氣及高氧中餵養，採用實時熒光定量PCR和蛋白質免疫印跡方法檢測第1、3、7天肺組織VEGF、Ang-1mRNA 和蛋白錶達水平，觀察肺組織形態結構的變化。結果：第7天VEGF、Ang-1 mRNA 相對含量，對照組為0．722±0．372、0．828±0．462，高氧組為0．239±0．293、0．327±0．184；VEGF、Ang-1蛋白相對含量，對照組為0．632±0．289、0．573±0．436，高氧組為0．358±0．128、0．204±0．068。第7天VEGF、Ang-1 mRNA 和蛋白錶達水平在對照組和高氧組比較差異均存在顯著性（P＜0．05）。光鏡下觀察高氧組肺組織錶現為肺組織顯著髮育不良，肺泡結構簡單化，肺泡數目減少和肺微血管髮育受阻。結論：VEGF和Ang-1是肺血管髮育過程中不可或缺的調節因子，參與瞭BPD病理髮生，對肺髮育起著重要作用。
목적：연구고양유도신생서급성폐손상 VEGF 화 Ang-1적표체급기대폐발육적영향，위림상방치지기관폐발육불량（BPD）제공실험의거。방법：2～3일령신생SD대서공48지수궤분조，우공기급고양중위양，채용실시형광정량PCR화단백질면역인적방법검측제1、3、7천폐조직VEGF、Ang-1mRNA 화단백표체수평，관찰폐조직형태결구적변화。결과：제7천VEGF、Ang-1 mRNA 상대함량，대조조위0．722±0．372、0．828±0．462，고양조위0．239±0．293、0．327±0．184；VEGF、Ang-1단백상대함량，대조조위0．632±0．289、0．573±0．436，고양조위0．358±0．128、0．204±0．068。제7천VEGF、Ang-1 mRNA 화단백표체수평재대조조화고양조비교차이균존재현저성（P＜0．05）。광경하관찰고양조폐조직표현위폐조직현저발육불량，폐포결구간단화，폐포수목감소화폐미혈관발육수조。결론：VEGF화Ang-1시폐혈관발육과정중불가혹결적조절인자，삼여료BPD병리발생，대폐발육기착중요작용。
Objective To investigate the expressions of VEGF and Ang-1 in neonatal rats with hyperoxia-induced BPD and its effects on lung development. Methods 48 SD neonatal rats (2 or 3 days old) were randomly divided into two group and raised under hyperoxia or air for 1, 3 and 7 days, respectively. Real-time RT-PCR and Western blot were used to determine the level VEGF and Ang-1 mRNAs and proteins in lung tissues of the two groups. HE staining was used to observe the changes of lung morphology. Results The levels of VEGF and Ang-1 mRNAs in the lung on the 7th day in the control group were 0.722 ± 0.372 and 0.828 ± 0.462, respectively, and those in hyperoxia group were 0.239 ± 0.293 and 0.327 ± 0.184 , respectively. The levels of VEGF and Ang-1 proteins on the 7th day in the control group were 0.632 ± 0.289 and 0.573 ± 0.436, respectively, and those in hyperoxia group were 0.358 ± 0.128 and 0.204 ± 0.068 , respectively. Comparing to the control group , the levels of VEGF and Ang-1 mRNAs and proteins on the 7th days significantly decreased in the hyperoxia groups (P<0.05). The lung tissues of the hyperoxia group display dysplastic features with , alveolar simplification , reduced alveolar numbers and retardation on microvascular development. Conclusion VEGF and Ang-1, functioning as an important regulators for pulmonary vascular development , are involved in the pathogenesis of BPD and the lung development.
