中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2014年
3期
280-283
,共4页
董蕾%张馨文%丛金鹏%于文成
董蕾%張馨文%叢金鵬%于文成
동뢰%장형문%총금붕%우문성
慢性阻塞性肺血管重塑%氧化物酶体增殖物激活受体γ%TOLL样受体4
慢性阻塞性肺血管重塑%氧化物酶體增殖物激活受體γ%TOLL樣受體4
만성조새성폐혈관중소%양화물매체증식물격활수체γ%TOLL양수체4
Chronic obstructive Pulmonary vascular remodeling%Peroxisome proliferator-activated receptorγ%Toll-like receptor 4
目的 探讨过氧化物酶体增殖物激活受体γ(Peroxisome proliferator-activated receptorγ PPAR-γ)及TOLL样受体4(Toll-like receptors TLR4)在慢性阻塞性肺病(COPD)肺血管重塑中的作用及相关性.方法 收集男性鳞癌手术患者90例,根据肺功能分为COPD组和对照组(非COPD组),各45例,取其肺叶切除后的无肿瘤浸润外周肺组织,HE染色光镜下观察血管炎症程度并测定管壁厚度占血管外径百分比(WT％)、血管壁横断面积占管径总面积百分比(WA％)；应用免疫组织化学方法检测肺动脉内皮细胞PPAR-γ及TLR4的表达情况.结果 对照组及COPD组中反应血管重建的WA％、WT％及肺动脉内皮细胞PPAR-γ、TLR4阳性表达率的比较差异均有统计学意义[WA％:(26.09±2.82)％与(43.98±6.43)％,t=-13.949 P=0.000; WT％:(15.57±1.75)％与(27.37±3.34)％,t=-7.140,P=0.000; PPAR-γ:(28.22±15.08)％与(10.74±8.81)％t =5.483,P =0.000;TLR4:(4.67±4.47)％与(31.41±14.67)％,t=-9.555,P=0.000].PPAR-γ与TLR4表达呈负相关(r=-0.404,P＜0.01).结论 COPD患者肺动脉炎细胞浸润,血管机化重塑,PPAR-γ、TLR4表达异常均参与COPD肺血管重塑的调控.
目的 探討過氧化物酶體增殖物激活受體γ(Peroxisome proliferator-activated receptorγ PPAR-γ)及TOLL樣受體4(Toll-like receptors TLR4)在慢性阻塞性肺病(COPD)肺血管重塑中的作用及相關性.方法 收集男性鱗癌手術患者90例,根據肺功能分為COPD組和對照組(非COPD組),各45例,取其肺葉切除後的無腫瘤浸潤外週肺組織,HE染色光鏡下觀察血管炎癥程度併測定管壁厚度佔血管外徑百分比(WT％)、血管壁橫斷麵積佔管徑總麵積百分比(WA％)；應用免疫組織化學方法檢測肺動脈內皮細胞PPAR-γ及TLR4的錶達情況.結果 對照組及COPD組中反應血管重建的WA％、WT％及肺動脈內皮細胞PPAR-γ、TLR4暘性錶達率的比較差異均有統計學意義[WA％:(26.09±2.82)％與(43.98±6.43)％,t=-13.949 P=0.000; WT％:(15.57±1.75)％與(27.37±3.34)％,t=-7.140,P=0.000; PPAR-γ:(28.22±15.08)％與(10.74±8.81)％t =5.483,P =0.000;TLR4:(4.67±4.47)％與(31.41±14.67)％,t=-9.555,P=0.000].PPAR-γ與TLR4錶達呈負相關(r=-0.404,P＜0.01).結論 COPD患者肺動脈炎細胞浸潤,血管機化重塑,PPAR-γ、TLR4錶達異常均參與COPD肺血管重塑的調控.
목적 탐토과양화물매체증식물격활수체γ(Peroxisome proliferator-activated receptorγ PPAR-γ)급TOLL양수체4(Toll-like receptors TLR4)재만성조새성폐병(COPD)폐혈관중소중적작용급상관성.방법 수집남성린암수술환자90례,근거폐공능분위COPD조화대조조(비COPD조),각45례,취기폐협절제후적무종류침윤외주폐조직,HE염색광경하관찰혈관염증정도병측정관벽후도점혈관외경백분비(WT％)、혈관벽횡단면적점관경총면적백분비(WA％)；응용면역조직화학방법검측폐동맥내피세포PPAR-γ급TLR4적표체정황.결과 대조조급COPD조중반응혈관중건적WA％、WT％급폐동맥내피세포PPAR-γ、TLR4양성표체솔적비교차이균유통계학의의[WA％:(26.09±2.82)％여(43.98±6.43)％,t=-13.949 P=0.000; WT％:(15.57±1.75)％여(27.37±3.34)％,t=-7.140,P=0.000; PPAR-γ:(28.22±15.08)％여(10.74±8.81)％t =5.483,P =0.000;TLR4:(4.67±4.47)％여(31.41±14.67)％,t=-9.555,P=0.000].PPAR-γ여TLR4표체정부상관(r=-0.404,P＜0.01).결론 COPD환자폐동맥염세포침윤,혈관궤화중소,PPAR-γ、TLR4표체이상균삼여COPD폐혈관중소적조공.
Objective To explore the role and correlation of peroxisome proliferator-activated receptor γ (PPAR-γ) and Toll-like receptor 4 (TLR4) on pulmonary vascular remodeling of chronic obstructive pulmonary disease(COPD).Methods Ninety male patients who underwent surgery for squamous cell carcinoma were enrolled as our subjects.All patients were divided into COPD group and uon-COPD group based on lung function,and 45 cases in each group.Peripheral lung tissues without tumor infiltrated after lobectomy were taken to assess the degree of arterial inflammation,percentage of wall thickness to vessel diamater (WT％) and percentage of wall area to total vascular area(WA％) were measured through Hematoxylin-Eosin(HE) staining under light microscope.The expression of PPARγand TLR4 were determined by immunohistochemistry.Results The distribution of WA％ and WT％ in COPD group were (43.98 ±6.43)％ and (27.37 ±3.34)％,higher than that of non-COPD group ((26.09 ± 2.82) ％,t =-13.949,P =0.000 ; (15.57 ± 1.75) ％,t =-7.140,P =0.000).The expression of PPAR-γand TLR4 in COPD group were (10.74 ± 8.81) ％,(3 1.41 ± 14.67) ％ respectively,and (28.22 ± 15.08)％,(4.67 ± 4.47)％ in non-COPD group.The differences were statistic significantly(t =5.483,P =0.000; t =-9.555,P =0.000).And there was negative correlation between the expression of PPARγand TLR4 (r =-0.404,P ＜ 0.01).Conclusion The pulmonary vascular of COPD patients showed the obviously inflammatory cell infiltration,fibrosis and proliferation,and PPAR-γ and TLR4 participate in the regulation of pulmonary vascular remodeling.
