CAJ | 학술논문

目的对两种组织工程支架的性能进行研究，为寻求更符合组织工程的支架提供实验依据。方法采用二次冻干技术制备CS-SF-TCP及CS支架，采用液体替代法等方法对两种支架的一般性能进行测定，将制备成一定体积的两种支架植入Wistar大鼠的背部，于2、4、6、周取出植入物行组织学染色观察。结果制备的两种支架成白色海绵状物质，孔隙率为70%-80%，孔径值为100-150μm。埋植支架周围皮下组织无充血、化脓等现象,无组织坏死发生,植入部位早期有轻度的炎症反应,后较快消退。植入物表变均被致密的薄层纤维膜包绕。组织染色结果可见支架的表面有细胞附着，两种支架内部孔隙失去连接，有断裂吸收现象。结论单纯CS支架在体内降解过快，CS-SF-TCP支架具有良好的降解性与组织相容性,是一种很有潜力的组织工程支架材料。
목적대량충조직공정지가적성능진행연구，위심구경부합조직공정적지가제공실험의거。방법채용이차동간기술제비CS-SF-TCP급CS지가，채용액체체대법등방법대량충지가적일반성능진행측정，장제비성일정체적적량충지가식입Wistar대서적배부，우2、4、6、주취출식입물행조직학염색관찰。결과제비적량충지가성백색해면상물질，공극솔위70%-80%，공경치위100-150μm。매식지가주위피하조직무충혈、화농등현상,무조직배사발생,식입부위조기유경도적염증반응,후교쾌소퇴。식입물표변균피치밀적박층섬유막포요。조직염색결과가견지가적표면유세포부착，량충지가내부공극실거련접，유단렬흡수현상。결론단순CS지가재체내강해과쾌，CS-SF-TCP지가구유량호적강해성여조직상용성,시일충흔유잠력적조직공정지가재료。
PURPOSE: The purpose of the study is to research the property about the chitosan scaffold and the chitosan-silk fibroin-tricalcium scaffold, in order to find the more suitable scaffold for tissue engineering. METHODS:The two scaffolds were prepared by freezing and lyophilizing taking water as the hole-making agent. The two scaffolds phosphate complex was implanted in the dorsal muscles of rats for assessment of in vitro and in vivo degradation as well as biocompatibility. RESULTS: These scaffolds phosphate complex was spongy, with uniform porosity and irregular <br> shapes of pores. The pores were connected with each other, with a porosity of 70%-80% and diameter of approximately 100-150μm. All the implants were covered by thick memberance. These scaffolds were loss the connect. Conclusion The chitosan-silk fibroin-tricalcium phosphate porous complex was more appropriate as the tissue engineering scaffold.