CAJ | 학술논문

目的探讨积雪草酸(asiatic acid，AA)对高糖环境中心肌细胞线粒体电压依赖性阴离子通道(voltage-dependent anion channel， VDAC)的表达和Ca2+超载的影响。方法将培养72小时的SD乳鼠心肌细胞随机分为三组：正常对照组(5mmol/L GS)、高糖培养组(25mmol/L GS)、高糖+AA组(25mmol/L GS+10、20、40、80nmol/L AA)，继续培养24小时后，Western blot法检测VDAC表达变化；WST法测定心肌细胞超氧化物歧化酶(SOD)活性，比色法测定细胞内丙二醛(MDA)含量；流式细胞仪检测心肌细胞内Ga2+含量。结果与对照组相比，高糖培养组心肌细胞线粒体VDAC表达量及SOD活性明显降低(P＜0.05)；细胞内Ca2+及MDA含量显著升高(P＜0.05)。加入AA处理后，VDAC表达量及SOD活性明显上升(P＜0.05)；细胞内MDA含量及胞内Ca2+含量显著降低(P＜0.05)；SOD活性与VDAC表达量呈正相关而与细胞内Ca2+呈负相关；MDA含量与VDAC表达呈负相关而与细胞内Ca2+呈正相关。结论 AA可能通过减轻线粒体氧化应激而提高线粒体VDAC表达量进而减轻细胞内Ca2+超载。
목적탐토적설초산(asiatic acid，AA)대고당배경중심기세포선립체전압의뢰성음리자통도(voltage-dependent anion channel， VDAC)적표체화Ca2+초재적영향。방법 장배양72소시적SD유서심기세포수궤분위삼조：정상대조조(5mmol/L GS)、고당배양조(25mmol/L GS)、고당+AA조(25mmol/L GS+10、20、40、80nmol/L AA)，계속배양24소시후，Western blot법검측VDAC표체변화；WST법측정심기세포초양화물기화매(SOD)활성，비색법측정세포내병이철(MDA)함량；류식세포의검측심기세포내Ga2+함량。결과 여대조조상비，고당배양조심기세포선립체VDAC표체량급SOD활성명현강저(P＜0.05)；세포내Ca2+급MDA함량현저승고(P＜0.05)。가입AA처리후，VDAC표체량급SOD활성명현상승(P＜0.05)；세포내MDA함량급포내Ca2+함량현저강저(P＜0.05)；SOD활성여VDAC표체량정정상관이여세포내Ca2+정부상관；MDA함량여VDAC표체정부상관이여세포내Ca2+정정상관。결론 AA가능통과감경선립체양화응격이제고선립체VDAC표체량진이감경세포내Ca2+초재。
Objective To investigate the effects of asiatic acid on the expression of voltage dependence anion channel (VDAC) and the influnce of Ca2+ overload in diabetic cardiomyopathy myocardial cell. Methods:The SD neonate rat myocardial cells were divided randomly into three groups after being cultured for 72 hours: control group(5mmol/L GS), high glucose culture group(25mmol/L GS), high glucose add AA group(25mmol/L GS+10、20、40、80nmol/L AA). After another 24 hours culture,the VDAC was determined by Western blot; intra-cellular superoxide dismutase(SOD) activity was measured by WST , and the content of malondialdehyde (MDA) was measured by colorimetry ; Flow cytometer was used to evaluate the Ca2+ of myocardial cells. Results:Compared to the control group, in the high glucose culture group, the VDAC in the myocardial cell mitochondria and the activity of SOD was obviously descended (P<0.05); The content of MDA and Ca2+ in myocardial cells was significantly increased (P<0.05). The expression of VDAC and the SOD activity were obviously increased (P<0.05) after being treated with AA, the content of MDA and Ca2+ was significant decreased(P<0.05). SOD activity was positively correlated with VDAC expresstion but negatively correlated with Ca2+. MDA was negatively correlated with VDAC but positively with Ca2+. Conclusion:AA could improve the VDAC expression in mitochondrial, reduce the oxidative stress of mitochondrial and reduce the intracellular Ca2+ overload.