中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2013年
11期
53-55
,共3页
克咳片%麻黄%盐酸麻黄碱%盐酸伪麻黄碱%含量测定%高效液相色谱法
剋咳片%痳黃%鹽痠痳黃堿%鹽痠偽痳黃堿%含量測定%高效液相色譜法
극해편%마황%염산마황감%염산위마황감%함량측정%고효액상색보법
Keke Tablets%ephedrae herba%ephedrinehydrochloride%pseudoephedrine hydrochlorid%content determination%HPLC
目的建立高效液相色谱法同时测定克咳片中盐酸麻黄碱、盐酸伪麻黄碱含量的方法。方法色谱柱：phenomenex Synergi Polar-RP柱(4.6 mm×250 mm,4μm)；流动相：甲醇∶0.092%磷酸溶液(含0.04%三乙胺和0.02%二正丁胺)＝1.5∶98.5；检测波长：210 nm；流速：1.0 mL/min；柱温：30℃。结果盐酸麻黄碱和盐酸伪麻黄碱的线性范围分别为6.51×10-3～0.651μg(r＝0.9999)、6.27×10-3～0.627μg(r＝1),平均加样回收率(n＝6)分别为102.26%、103.71%,RSD 分别为0.34%、0.22%。结论该方法简便、准确,重复性好,专属性高,结果可靠,可有效控制克咳片中生物碱成分的质量。
目的建立高效液相色譜法同時測定剋咳片中鹽痠痳黃堿、鹽痠偽痳黃堿含量的方法。方法色譜柱：phenomenex Synergi Polar-RP柱(4.6 mm×250 mm,4μm)；流動相：甲醇∶0.092%燐痠溶液(含0.04%三乙胺和0.02%二正丁胺)＝1.5∶98.5；檢測波長：210 nm；流速：1.0 mL/min；柱溫：30℃。結果鹽痠痳黃堿和鹽痠偽痳黃堿的線性範圍分彆為6.51×10-3～0.651μg(r＝0.9999)、6.27×10-3～0.627μg(r＝1),平均加樣迴收率(n＝6)分彆為102.26%、103.71%,RSD 分彆為0.34%、0.22%。結論該方法簡便、準確,重複性好,專屬性高,結果可靠,可有效控製剋咳片中生物堿成分的質量。
목적건립고효액상색보법동시측정극해편중염산마황감、염산위마황감함량적방법。방법색보주：phenomenex Synergi Polar-RP주(4.6 mm×250 mm,4μm)；류동상：갑순∶0.092%린산용액(함0.04%삼을알화0.02%이정정알)＝1.5∶98.5；검측파장：210 nm；류속：1.0 mL/min；주온：30℃。결과염산마황감화염산위마황감적선성범위분별위6.51×10-3～0.651μg(r＝0.9999)、6.27×10-3～0.627μg(r＝1),평균가양회수솔(n＝6)분별위102.26%、103.71%,RSD 분별위0.34%、0.22%。결론해방법간편、준학,중복성호,전속성고,결과가고,가유효공제극해편중생물감성분적질량。
Objective To establish the method for determining ephedrine hydochloride and pseudoephedrine hydrochloride in Keke Tablets by HPLC. Methods The samples were analyzed by a phemomenex Synergi Polar-RP column (4.6 mm×250 mm, 4 μm), with mobile phase methanol∶0.092%phosphoric acid (0.04%triethylamine and 0.02%second butylamine)=1.5∶98.5 at flow rate of 1.0 mL/min and detection at UV wavelength of 210 nm. The column temperature was 30 ℃. Results The linear ranges of ephedrine hydrochloride and pseudoephedrine hydrochloride were 6.51×10-3-0.651 μg (r=0.999 9) and 6.27×10-3-0.627 μg (r=1), respectively. The average recoveries (n=6) of ephedrine hydrochloride and pseudoephedrine hydrochloride were 102.26%and 103.71%, with RSD of 0.34%and 0.22%, respectively. Conclusion This method is simple, accurate, reproducible, highly specific and reliable results, and has the ability to effectively control the quality of alkaloids in Keke Tablets.
