中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2013年
11期
49-50
,共2页
十八味诃子利尿丸%没食子酸%高效液相色谱法%含量测定
十八味訶子利尿汍%沒食子痠%高效液相色譜法%含量測定
십팔미가자이뇨환%몰식자산%고효액상색보법%함량측정
18 Taste Myrobalan Diuretic Pill%gallic acid%HPLC%content determination
目的建立十八味诃子利尿丸中没食子酸的高效液相色谱含量测定方法。方法采用ODS-2 HYPERSIL C18柱(250 mm×4.6 mm,5μm)；流动相：甲醇-0.1%磷酸溶液(7∶93)；流速：1.0 mL/min；检测波长：270 nm；柱温：30℃。结果没食子酸对照品进样量在0.063～0.315μg范围内有良好的线性关系,Y﹦2902.1X-5.95,r＝0.9996,平均回收率为98.56%,RSD＝0.41%(n＝5)。结论本法灵敏、简便、准确,重复性好,可用于该制剂的质量控制。
目的建立十八味訶子利尿汍中沒食子痠的高效液相色譜含量測定方法。方法採用ODS-2 HYPERSIL C18柱(250 mm×4.6 mm,5μm)；流動相：甲醇-0.1%燐痠溶液(7∶93)；流速：1.0 mL/min；檢測波長：270 nm；柱溫：30℃。結果沒食子痠對照品進樣量在0.063～0.315μg範圍內有良好的線性關繫,Y﹦2902.1X-5.95,r＝0.9996,平均迴收率為98.56%,RSD＝0.41%(n＝5)。結論本法靈敏、簡便、準確,重複性好,可用于該製劑的質量控製。
목적건립십팔미가자이뇨환중몰식자산적고효액상색보함량측정방법。방법채용ODS-2 HYPERSIL C18주(250 mm×4.6 mm,5μm)；류동상：갑순-0.1%린산용액(7∶93)；류속：1.0 mL/min；검측파장：270 nm；주온：30℃。결과몰식자산대조품진양량재0.063～0.315μg범위내유량호적선성관계,Y﹦2902.1X-5.95,r＝0.9996,평균회수솔위98.56%,RSD＝0.41%(n＝5)。결론본법령민、간편、준학,중복성호,가용우해제제적질량공제。
Objective To establish the method for determination of gallic acid in Shibawei Heziliniao Pill by HPLC. Methods ODS-2 HYPERSIL C18 column (250 mm×4.6 mm, 5 μm) was used, with mobile phase of methanol-0.1%phosphoric acid solution (7∶93). The flow rate was 1.0 mL/min, the detection wavelength was 270 nm, and the column temperature was 30 ℃. Results Gallic acid showed a good linear relationship within the range of 0.063-0.315 μg, Y=2902.1X-5.95, r=0.999 6. The average recovery was 98.56%, and RSD was 0.41% (n=5). Conclusion The method is sensitive, simple, accurate, reproducible, and can be used for quality control of 18 Taste Myrobalan Diuretic Pill.
