CAJ | 학술논문

目的建立健脾复肝丸的质量标准。方法采用薄层色谱法对当归、白术进行定性鉴别。采用高效液相色谱法测定阿魏酸含量。色谱柱：TIANHE C18(250 mm×4.6 mm,5μm)；流动相：乙腈-0.085%磷酸(17∶83)；流速：1.0 mL/min；柱温：25℃；检测波长：323 nm。结果健脾复肝丸中当归、白术的供试品色谱中,在与对照品色谱相应位置上显相同颜色斑点。阿魏酸对照品在0.114～1.482μg范围内线性关系良好,平均加样回收率为98.36%, RSD＝1.16%。结论本方法简便准确、灵敏度高,可用于健脾复肝丸的质量控制。
목적건립건비복간환적질량표준。방법채용박층색보법대당귀、백술진행정성감별。채용고효액상색보법측정아위산함량。색보주：TIANHE C18(250 mm×4.6 mm,5μm)；류동상：을정-0.085%린산(17∶83)；류속：1.0 mL/min；주온：25℃；검측파장：323 nm。결과건비복간환중당귀、백술적공시품색보중,재여대조품색보상응위치상현상동안색반점。아위산대조품재0.114～1.482μg범위내선성관계량호,평균가양회수솔위98.36%, RSD＝1.16%。결론본방법간편준학、령민도고,가용우건비복간환적질량공제。
Objective To establish the quality standard for Jianpi Fugan Pills. Methods Angelicae Sinenis Radix and Atractylodis Macrocephalae Rhizome in the formulation were qualitatively identified by TLC. The content of ferulic acid was analyzed by HPLC. TIANHE C18 column (250 mm×4.6 mm, 5 μm) was used with a mobile phase of acetonitrile-0.085% phosphorous acid (17∶83). The content was detected at wavelength of 323 nm. The flow rate was 1.0 mL/min, and temperature was 25 ℃. Results The spots in the TLC of Angelicae Sinenis Radix and Atractylodis Macrocephalae Rhizome were in the same chormatograms of control articles. The linear range of ferulic acid was 0.114-1.482 μg. The average recovery was 98.36%and RSD was 1.16%. Conclusion The method is simple, accurate and highly sensitive, which can be used for quality control of Jianpi Fugan Pills.