CAJ | 학술논문

目的探讨黄芪当归配伍对气虚血瘀证大鼠血流动力学的改善及对Th1/Th2细胞的调节作用。方法采用限食＋强迫游泳＋皮下注射肾上腺素方法造成大鼠气虚血瘀证模型,分别用不同比例黄芪当归配伍灌胃给药治疗21 d。用血流变仪测定血液流变学指标,ELISA法测定血清中干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)含量。结果服药21 d后,与模型组比较,芪归1∶1组、芪归3∶1组、芪归5∶1组血流变低切指标明显减小,血黏度明显改善。模型组大鼠脾指数、胸腺指数均明显减小,而芪归5∶1组服药后脾指数明显上升。模型组 IFN-γ降低,IL-4升高,以Th2细胞反应占优势；芪归3∶1组、芪归5∶1组能升高血清IFN-γ含量,芪归5∶1组能降低IL-4含量。结论黄芪当归配伍对气虚血瘀证大鼠模型具有改善血液流变及增强免疫作用,并且可以调节Th1/Th2细胞平衡。其中以黄芪比例大于当归时效果较好,综合作用芪归5∶1组最佳。
목적탐토황기당귀배오대기허혈어증대서혈류동역학적개선급대Th1/Th2세포적조절작용。방법채용한식＋강박유영＋피하주사신상선소방법조성대서기허혈어증모형,분별용불동비례황기당귀배오관위급약치료21 d。용혈류변의측정혈액류변학지표,ELISA법측정혈청중간우소-γ(IFN-γ)、백세포개소-4(IL-4)함량。결과복약21 d후,여모형조비교,기귀1∶1조、기귀3∶1조、기귀5∶1조혈류변저절지표명현감소,혈점도명현개선。모형조대서비지수、흉선지수균명현감소,이기귀5∶1조복약후비지수명현상승。모형조 IFN-γ강저,IL-4승고,이Th2세포반응점우세；기귀3∶1조、기귀5∶1조능승고혈청IFN-γ함량,기귀5∶1조능강저IL-4함량。결론황기당귀배오대기허혈어증대서모형구유개선혈액류변급증강면역작용,병차가이조절Th1/Th2세포평형。기중이황기비례대우당귀시효과교호,종합작용기귀5∶1조최가。
Objective To observe the effects of the compatibility of Radix Astragali and Radix Angelicae Sinensis in different proportion on hemodynamic and balance of Th1/Th2 cells of Qi deficiency and blood stasis rat model. Methods Qi deficiency and blood stasis rats were caused by restricted diet, forced swimming and norepinephrine subcutaneous injecting, and treated by compatibility of Radix Astragali and Radix Angelicae Sinensis in different proportion of 5∶1, 3∶1, 1∶1 and 1∶2 for 21 days. The indexes of hemorheology were detected with hemorheological analyser, and the level of IFN-γand IL-4 in serum were tested by ELISA. Results Compared with model group, the compatibility of Radix Astragali and Radix Angelicae Sinensis in proportion of 1∶1, 3∶1 and 5∶1 groups reduced the low shear blood viscosity. The spleen index of model group decreased, and compatibility of Radix Astragali and Radix Angelicae Sinensis in 5∶1 proportion group increased spleen index. The level of IL-4 increased and IFN-γ decreased in the serum of model group, the compatibility of Radix Astragali and Radix Angelicae Sinensis in proportion of 3∶1 group increased the level of IFN-γ. Astragalus angelica 5∶1 group decreased the level of IL-4 and increased the level of IFN-γ. Conclusion The compatibility of Radix Astragali and Radix Angelicae Sinensis can improve hemorheology, adjust the balance of Th1/Th2 cells of Qi deficiency and blood stasis rats. The effects were better when Radix Astragali’s dosage greater than that of Radix Angelicae Sinensis, and the group of 5∶1 proportion was the best.