CAJ | 학술논문

目的观察对药陈皮半夏对颈动脉粥样硬化家兔血管细胞黏附分子-1(VCAM-1)及基质金属蛋白酶-9(MMP-9)的干预作用,探讨抗动脉硬化的作用机制。方法将25只雄性新西兰白兔随机分为正常组、模型组和中药大、中、小剂量组。正常组给予基础饲料喂饲,模型组和中药组予高脂饲料喂饲,并在喂饲1周后实施颈动脉内膜空气干燥术,术后中药组按10、8、6 mL/kg剂量予陈皮半夏煎剂灌胃,每日1次。4周后处死家兔,取每只家兔右侧颈动脉远端1 cm,固定后行HE染色,观察病理改变。应用免疫组化SABC法检测VCAM-1和MMP-9表达情况。结果模型组血管壁泡沫细胞、平滑肌细胞和内皮细胞的胞浆中有VCAM-1和MMP-9强阳性表达,显著高于正常组(P＜0.01)。中药组细胞内VCAM-1和MMP-9阳性表达显著低于模型组(P＜0.01),且随剂量的增加,陈皮半夏煎剂对VCAM-1和MMP-9的抑制逐渐增强。结论对药陈皮半夏可以有效抑制颈动脉硬化家兔VCAM-1和MMP-9的表达,提示其可能是通过抑制血管炎症反应、稳定斑块而发挥抗颈动脉硬化作用的。
목적관찰대약진피반하대경동맥죽양경화가토혈관세포점부분자-1(VCAM-1)급기질금속단백매-9(MMP-9)적간예작용,탐토항동맥경화적작용궤제。방법장25지웅성신서란백토수궤분위정상조、모형조화중약대、중、소제량조。정상조급여기출사료위사,모형조화중약조여고지사료위사,병재위사1주후실시경동맥내막공기간조술,술후중약조안10、8、6 mL/kg제량여진피반하전제관위,매일1차。4주후처사가토,취매지가토우측경동맥원단1 cm,고정후행HE염색,관찰병리개변。응용면역조화SABC법검측VCAM-1화MMP-9표체정황。결과모형조혈관벽포말세포、평활기세포화내피세포적포장중유VCAM-1화MMP-9강양성표체,현저고우정상조(P＜0.01)。중약조세포내VCAM-1화MMP-9양성표체현저저우모형조(P＜0.01),차수제량적증가,진피반하전제대VCAM-1화MMP-9적억제축점증강。결론대약진피반하가이유효억제경동맥경화가토VCAM-1화MMP-9적표체,제시기가능시통과억제혈관염증반응、은정반괴이발휘항경동맥경화작용적。
Objective To research the influence of tangerine peel and pinellia ternata on the expression of VCAM-1 and MMP-9 in carotid atherosclerosis rabbits, and explore its mechanism. Methods Totally 25 male rabbits were divided into control group, model group and Chinese medicine group. Control group was treated with basic diet, model group and Chinese medicine group were treated with both high-fat diet and carotid artery intima-air drying technique. Chinese medicine decoction group was treated with tangerine peel and pinellia ternata decoction, as 10, 8, 6 mL/kg, for 4 weeks. Then the right carotid was cut and fixed, and pathological changes were observed by HE staining. The expressions of VCAM-1 and MMP-9 were observed by immunol histochemistry SABC method. Results Compared with control group, the expression of VCAM-1 and MMP-9 in model group was significantly increased (P<0.01). And compared with model group, tangerine peel and pinellia ternata decoction significantly restrained VCAM-1 and MMP-9 expression (P<0.05). Conclusion Tangerine peel and pinellia ternata could restrained VCAM-1 and MMP-9 expression in carotid atherosclerosis rabbits, suggesting that its mechanism may be related with inhibiting vascular inflammatory reaction and stabilizing plaques.