CAJ | 학술논문

目的观察康欣胶囊对兔动脉粥样硬化(AS)血管斑块稳定性的影响,探讨其稳定斑块的可能机制。方法将40只纯种雄性新西兰大耳白兔随机分为模型组、阿托伐他汀钙组(简称他汀组)、康欣胶囊低剂量组(简称小剂量组)、康欣胶囊高剂量组(简称大剂量组)、正常对照组,每组8只。除正常组外,采用内膜损伤加高胆固醇饮食法建立兔AS模型,分别予相应处理,6周后进行主动脉血管病理形态学观察,采用免疫组化法检测基质金属蛋白酶-2(MMP-2)、血管细胞黏附分子-1(VCAM-1)在动脉斑块内的表达。结果康欣胶囊干预组动物血管腔壁比(W/L)、平均光密度、阳性面积百分比均较模型组小,动脉壁组织MMP-2及VCAM-1均较模型组下调,差异有统计学意义(P＜0.05,P＜0.01),以大剂量组最为明显。结论康欣胶囊可减少MMP-2、VCAM-1等炎性介质表达,可能是其稳定斑块的机制。
목적관찰강흔효낭대토동맥죽양경화(AS)혈관반괴은정성적영향,탐토기은정반괴적가능궤제。방법장40지순충웅성신서란대이백토수궤분위모형조、아탁벌타정개조(간칭타정조)、강흔효낭저제량조(간칭소제량조)、강흔효낭고제량조(간칭대제량조)、정상대조조,매조8지。제정상조외,채용내막손상가고담고순음식법건립토AS모형,분별여상응처리,6주후진행주동맥혈관병리형태학관찰,채용면역조화법검측기질금속단백매-2(MMP-2)、혈관세포점부분자-1(VCAM-1)재동맥반괴내적표체。결과강흔효낭간예조동물혈관강벽비(W/L)、평균광밀도、양성면적백분비균교모형조소,동맥벽조직MMP-2급VCAM-1균교모형조하조,차이유통계학의의(P＜0.05,P＜0.01),이대제량조최위명현。결론강흔효낭가감소MMP-2、VCAM-1등염성개질표체,가능시기은정반괴적궤제。
Objective To investigate the effect of Kangxin Capsule on the stability of atherosclerotic plaque in rabbits. Methods Forty purebred male New Zealand white rabbits were randomly divided into model group, atorvastatin group, Kangxin Capsule high and low dose groups, and normal control group, 8 rabbit for each group, and were given corresponding administration. Atherosclerosis model was made by intimal injury and high cholesterol diet except for normal control group. After 6 W, aortic vascular pathomorphology were observed, and immunohistochemical method was used to detect the expression of MMP-2 and VCAM-1 in atherosclerotic plaques. Results Compared with model group, the ratio of the thickness of wall to the area of lumina (W/L), average optical density, and the percentage of positive area of Kangxin Capsule groups were less, also, the expression of MMP-2 and VCAM-1 were reduced. The differences were significant (P<0.05 or P<0.01), especially in the high-dose group. Conclusion Kangxin Capsule can reduce the expression of MMP-2 and VCAM-1, which is the possible mechanism of stabilizing the atherosclerotic plaque.