CAJ | 학술논문

目的观察姜黄素对经肿瘤坏死因子-α(TNF-α)诱导后人肝癌细胞(Hepg-2)核转录因子(NF-κB)及其抑制蛋白-Bα(Iκ-Bα)表达的影响,探讨其对非酒精性脂肪性肝炎的影响。方法将Hepg-2细胞用含10%胎牛血清的DMEM培养基培养,细胞分为6组,即正常对照组、TNF-α组、2.5μmol/L姜黄素组、5μmol/L姜黄素组、10μmol/L姜黄素组、20μmol/L姜黄素组。MTT检测姜黄素对Hepg-2细胞增殖活力,Western blotting法检测NF-κB及IκB-Bα蛋白的变化。结果 Hepg-2细胞经 TNF-α刺激后,NF-κB 表达增强,与正常对照组比较差异有统计学意义(P＜0.05),而IκB-α表达虽增加但与正常对照组比较差异无统计学意义；经姜黄素干预后,NF-κB表达明显减弱,而IκB-Bα表达明显增强,与TNF-α组比较差异有统计学意义(P＜0.05)。结论姜黄素可拮抗TNF-α诱导的NF-κB炎性信号通路的激活,从而减轻肝细胞的炎性损伤。
목적관찰강황소대경종류배사인자-α(TNF-α)유도후인간암세포(Hepg-2)핵전록인자(NF-κB)급기억제단백-Bα(Iκ-Bα)표체적영향,탐토기대비주정성지방성간염적영향。방법장Hepg-2세포용함10%태우혈청적DMEM배양기배양,세포분위6조,즉정상대조조、TNF-α조、2.5μmol/L강황소조、5μmol/L강황소조、10μmol/L강황소조、20μmol/L강황소조。MTT검측강황소대Hepg-2세포증식활력,Western blotting법검측NF-κB급IκB-Bα단백적변화。결과 Hepg-2세포경 TNF-α자격후,NF-κB 표체증강,여정상대조조비교차이유통계학의의(P＜0.05),이IκB-α표체수증가단여정상대조조비교차이무통계학의의；경강황소간예후,NF-κB표체명현감약,이IκB-Bα표체명현증강,여TNF-α조비교차이유통계학의의(P＜0.05)。결론강황소가길항TNF-α유도적NF-κB염성신호통로적격활,종이감경간세포적염성손상。
Objective To investigate the effect of curcumin on the expression of nuclear transcription factor (NF-kappa B) and its inhibitory protein (Iκ-Bα) in liver cancer cells (Hepg-2) induced by TNF-α, and explore its effect on non-alcoholic steatohepatitis. Methods The cells were divided into normal group, TNF-alpha group, and different concentrations of curcumin treatment group. MTT and Western blotting were used to assay curcumin’s effect on Hepg-2 proliferation activity and changes of NF-κB and Iκ-Bα’s in Hepg-2. Results Compared with the normal group, TNF-αgroup enhanced NF-κB and Iκ-Bαexpression, but the increase of Iκ-Bα had no statistical significance (P>0.05). Curcumin treatment group’s NF-κB expression was significantly weakened than TNF-α group, while Iκ-Bα was significantly enhanced than that of TNF-α group. Conclusion Curcumin can antagonize NF-κB inflammatory signaling pathway activation induced by TNF-α, thus reduce the inflammatory injury of the liver cells.