CAJ | 학술논문

目的探讨子宫内膜异位症(EM)性不孕患者的中医辨证分型与腹腔液细胞因子白细胞介素(IL)-6、IL-8及肿瘤坏死因子-α(TNF-α)水平之间的相关性,为临床治疗提供思路。方法选取EM性不孕患者105例,辨证分为肾虚血瘀、气滞血瘀、热郁血瘀、寒凝血瘀、气虚血瘀、痰湿瘀阻六证型组。同时选取非EM性不孕患者30例作为对照组。采用酶联免疫吸附法测定腹腔液中IL-6、IL-8及TNF-α等细胞因子的水平。结果在105例EM性不孕患者中,肾虚血瘀证35例(33.33%),寒凝血瘀证22例(20.95%),气滞血瘀证18例(17.14%),热郁血瘀证10例(9.52%),气虚血瘀证11例(10.48%),痰湿瘀阻证9例(8.57%)。各证型组IL-6、IL-8、TNF-α水平均高于对照组(P＜0.01)。肾虚血瘀组IL-6水平高于其他各证型组(P＜0.05)；肾虚血瘀组、热郁血瘀组IL-8、TNF-α高于其他各组(P＜0.05)；肾虚血瘀组IL-8与热郁血瘀组比较,P＜0.05。结论 EM性不孕患者中医证型与腹腔液细胞因子IL-6、IL-8、TNF-α水平有一定的相关性,可以指导临床认识和治疗EM性不孕。
목적탐토자궁내막이위증(EM)성불잉환자적중의변증분형여복강액세포인자백세포개소(IL)-6、IL-8급종류배사인자-α(TNF-α)수평지간적상관성,위림상치료제공사로。방법선취EM성불잉환자105례,변증분위신허혈어、기체혈어、열욱혈어、한응혈어、기허혈어、담습어조륙증형조。동시선취비EM성불잉환자30례작위대조조。채용매련면역흡부법측정복강액중IL-6、IL-8급TNF-α등세포인자적수평。결과재105례EM성불잉환자중,신허혈어증35례(33.33%),한응혈어증22례(20.95%),기체혈어증18례(17.14%),열욱혈어증10례(9.52%),기허혈어증11례(10.48%),담습어조증9례(8.57%)。각증형조IL-6、IL-8、TNF-α수평균고우대조조(P＜0.01)。신허혈어조IL-6수평고우기타각증형조(P＜0.05)；신허혈어조、열욱혈어조IL-8、TNF-α고우기타각조(P＜0.05)；신허혈어조IL-8여열욱혈어조비교,P＜0.05。결론 EM성불잉환자중의증형여복강액세포인자IL-6、IL-8、TNF-α수평유일정적상관성,가이지도림상인식화치료EM성불잉。
Objective To discuss the relationship between TCM syndromes and peritoneal fluid cytokine IL-6, IL-8 and TNF-α of endometriosis infertility patients, and provide thoughts for clinical treatment. Methods Totally 105 patients of endometriosis infertility were differentiated to six syndrome groups, including kidney deficiency and blood stasis, qi stagnation and blood stasis, heat stagnation and blood stasis, cold congelation and blood stasis, qi deficiency and blood stasis, phlegm-dampness stagnation. Thirty patients of non-endometriosis infertility were selected as control group. Enzyme-linked immunosorbent assay was used to detect cytokine levels of IL-6, IL-8 and TNF-α. Results In 105 cases of EM infertility, 35 cases (33.33%) were syndrome of kidney deficiency and blood stasis, 22 cases (20.95%) were syndrome of cold congelation and blood stasis, 18 cases (17.14%) were syndrome of qi stagnation and blood stasis, 10 cases (9.52%) were syndrome of heat stagnation and blood stasis, 11 cases (10.48%) were syndrome of qi deficiency and blood stasis, 9 cases (8.57%) were phlegm-dampness stagnation syndrome. The levels of IL-6, IL-8 and TNF-αin each syndrome group of EM infertility were higher than control group (P<0.01). IL-6 level in kidney deficiency and blood stasis group were higher than other syndrome groups (P<0.05). The levels of IL-8 and TNF-α in kidney deficiency and blood stasis group, and heat stagnation and blood stasis group were higher than other syndrome groups (P<0.05). IL-8 in kidney deficiency and blood stasis group was higher than that in heat stagnation and blood stasis group (P<0.05). Conclusion TCM syndromes in patients with endometriosis infertility have some relevance with peritoneal fluid cytokine levels of IL-6, IL-8, TNF-α, which may guide clinical understanding and treatment of endometriosis infertility.