中国兽药杂志
中國獸藥雜誌
중국수약잡지
CHINESE JOURNAL OF VETERINARY DRUG
2014年
5期
43-46
,共4页
韩立%班付国%周红霞%王林%张闪烁%王丽
韓立%班付國%週紅霞%王林%張閃爍%王麗
한립%반부국%주홍하%왕림%장섬삭%왕려
高效液相色谱法%穿心莲注射液%穿心莲内酯%脱水穿心莲内酯
高效液相色譜法%穿心蓮註射液%穿心蓮內酯%脫水穿心蓮內酯
고효액상색보법%천심련주사액%천심련내지%탈수천심련내지
HPLC%Chuanxinlian injection%andrographolide%dehydroandrographolide
建立了穿心莲注射液中穿心莲内酯和脱水穿心莲内酯含量测定的高效液相色谱方法。采用Waters XBridge C18色谱柱（150 mm×4．6 mm，5μm），甲醇：水（65∶35）为流动相，流速为1．0 mL／min，穿心莲内酯波长为225 nm，脱水穿心莲内酯波长为254 nm，柱温30℃。穿心莲内酯和脱水穿心莲内酯均在0．01～0．2 mg／mL浓度范围内线性关系良好。系统精密度分别为0．35％和0．30％，平均回收率分别为96．57％和97．98％。结果表明，该方法简单可行、重复性好，可用于穿心莲注射液中有效成分的含量测定。
建立瞭穿心蓮註射液中穿心蓮內酯和脫水穿心蓮內酯含量測定的高效液相色譜方法。採用Waters XBridge C18色譜柱（150 mm×4．6 mm，5μm），甲醇：水（65∶35）為流動相，流速為1．0 mL／min，穿心蓮內酯波長為225 nm，脫水穿心蓮內酯波長為254 nm，柱溫30℃。穿心蓮內酯和脫水穿心蓮內酯均在0．01～0．2 mg／mL濃度範圍內線性關繫良好。繫統精密度分彆為0．35％和0．30％，平均迴收率分彆為96．57％和97．98％。結果錶明，該方法簡單可行、重複性好，可用于穿心蓮註射液中有效成分的含量測定。
건립료천심련주사액중천심련내지화탈수천심련내지함량측정적고효액상색보방법。채용Waters XBridge C18색보주（150 mm×4．6 mm，5μm），갑순：수（65∶35）위류동상，류속위1．0 mL／min，천심련내지파장위225 nm，탈수천심련내지파장위254 nm，주온30℃。천심련내지화탈수천심련내지균재0．01～0．2 mg／mL농도범위내선성관계량호。계통정밀도분별위0．35％화0．30％，평균회수솔분별위96．57％화97．98％。결과표명，해방법간단가행、중복성호，가용우천심련주사액중유효성분적함량측정。
A HPLC method was established for the determination of andrographolide and dehydroandrographolide in Chuanxinlian injection. A Waters XBridge C18 chromatographic column (150 mm×4.6 mm, 5μm) was used, with the mobile phase of methanol-water(65 ∶ 35) at the flow rate of 1.0 mL/min. The detection wavelength of andrographolide and dehydroandrographolide were respectively 225 nm and 254 nm, and the column temperature was 30 ℃. Both andrographolide and dehydroandrographolide had good linearity within the concentration range of 0.01~0. 2 mg/mL. The system precision were 0. 35% and 0. 30%, and the average recovery were 96. 57% and 97.98% respectively. The method is simple, feasible and repeatable, it can be used as quality control for Chuanxinlian injection.
