中华临床医师杂志(电子版)
中華臨床醫師雜誌(電子版)
중화림상의사잡지(전자판)
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
12期
5378-5381
,共4页
魏元元%张绍维%王巍%李鹏飞%张玉敏%罗雨葳%赵文州
魏元元%張紹維%王巍%李鵬飛%張玉敏%囉雨葳%趙文州
위원원%장소유%왕외%리붕비%장옥민%라우위%조문주
糖尿病,2型%烟酰胺磷酸核糖基转移酶%葡萄糖转运体4型%1-磷脂酰肌醇3-激酶
糖尿病,2型%煙酰胺燐痠覈糖基轉移酶%葡萄糖轉運體4型%1-燐脂酰肌醇3-激酶
당뇨병,2형%연선알린산핵당기전이매%포도당전운체4형%1-린지선기순3-격매
Diabetes mellitus,type 2%Nicotinamide phosphoribosyltransferase%Glucose transporter type 4%1-phosphatidylinositol 3-kinase
目的研究内脂素对糖尿病大鼠骨骼肌葡萄糖转运蛋白-4( GLUT4)和磷脂酰肌醇-3-激酶(PI3K)表达的影响并探讨其机制。方法健康雄性Wistar大鼠随机分为普通饲料组(N组)、高脂饲料组(H组)、普通饲料糖尿病模型组(N+S组)及高脂饲料糖尿病模型组(H+S组)。糖尿病模型组大鼠腹腔注射链脲佐菌素造模。造模成功8周后,两组糖尿病大鼠再分别随机分出一组为V+N+S组和V+H+S组,腹腔注射内脂素重组蛋白。采用实时定量RT-PCR技术检测骨骼肌GLUT4 mRNA表达, Western blot 技术检测GLUT4和PI3K蛋白表达。结果 N+S组与N组、H+S组与H组大鼠骨骼肌GLUT4 mRNA,GLUT4和PI3K蛋白表达水平比较明显减低( P<0.05)。 V+N+S组与N+S组、V+H+S组与H+S组比较均有所升高(P<0.05)。结论内脂素可能通过增加PI3K的蛋白表达影响GLUT4的基因表达,导致其蛋白表达增加,从而降低血糖。
目的研究內脂素對糖尿病大鼠骨骼肌葡萄糖轉運蛋白-4( GLUT4)和燐脂酰肌醇-3-激酶(PI3K)錶達的影響併探討其機製。方法健康雄性Wistar大鼠隨機分為普通飼料組(N組)、高脂飼料組(H組)、普通飼料糖尿病模型組(N+S組)及高脂飼料糖尿病模型組(H+S組)。糖尿病模型組大鼠腹腔註射鏈脲佐菌素造模。造模成功8週後,兩組糖尿病大鼠再分彆隨機分齣一組為V+N+S組和V+H+S組,腹腔註射內脂素重組蛋白。採用實時定量RT-PCR技術檢測骨骼肌GLUT4 mRNA錶達, Western blot 技術檢測GLUT4和PI3K蛋白錶達。結果 N+S組與N組、H+S組與H組大鼠骨骼肌GLUT4 mRNA,GLUT4和PI3K蛋白錶達水平比較明顯減低( P<0.05)。 V+N+S組與N+S組、V+H+S組與H+S組比較均有所升高(P<0.05)。結論內脂素可能通過增加PI3K的蛋白錶達影響GLUT4的基因錶達,導緻其蛋白錶達增加,從而降低血糖。
목적연구내지소대당뇨병대서골격기포도당전운단백-4( GLUT4)화린지선기순-3-격매(PI3K)표체적영향병탐토기궤제。방법건강웅성Wistar대서수궤분위보통사료조(N조)、고지사료조(H조)、보통사료당뇨병모형조(N+S조)급고지사료당뇨병모형조(H+S조)。당뇨병모형조대서복강주사련뇨좌균소조모。조모성공8주후,량조당뇨병대서재분별수궤분출일조위V+N+S조화V+H+S조,복강주사내지소중조단백。채용실시정량RT-PCR기술검측골격기GLUT4 mRNA표체, Western blot 기술검측GLUT4화PI3K단백표체。결과 N+S조여N조、H+S조여H조대서골격기GLUT4 mRNA,GLUT4화PI3K단백표체수평비교명현감저( P<0.05)。 V+N+S조여N+S조、V+H+S조여H+S조비교균유소승고(P<0.05)。결론내지소가능통과증가PI3K적단백표체영향GLUT4적기인표체,도치기단백표체증가,종이강저혈당。
Objective To investigate the effect of visfatin on GLUT4 mRNA expression and PI3K protein expression in skeletal muscle of diabetic rats and explore its mechanism .Methods Wistar rats were randomly divided into four groups:normal diet feeding group ( N ) , high-fat diet feeding group ( H ) , normal diet feeding +streptozotocin(STZ)-induced diabetic group(N+S)and high-fat diet feeding+STZ-induced diabetic group(H+S). Diabetic rats were given intraperitoneal injection of small dose STZ to establish models of type 2 diabetes.Visfatin were intraperitoneally injected in partial rats(V+N+S,V+H+S)from group(N+S)and group(H+S)after 8 weeks of establishment of models .Western blotting were used to assess the protein expression of PI 3K and GLUT4. GLUT4 mRNA expression were measured with real time RT-PCR.Results GLUT4 mRNA levels,GLUT4 and PI3K protein expression were decreased(P<0.05)in the Group(N+S)compared with group N,and in the Group(H+S) compared with group H,and increased(P<0.05)in the Group(V+N+S)compared with group(N+S),and in the Group(V+H+S)compared with group(H+S)respectively.Conclusion Our data show that visfatin may increase GLUT4 mRNA levels and protein expression and lower plasma glucose levels by increase PI 3K protein expression in skeletal muscle of diabetic rats .