CAJ | 학술논문

目的探讨 miR-195过表达对体外培养的人脑胶质瘤细胞U251和 SHG-44增殖的影响。方法通过脂质体将miR-195 mimics转染至U251和SHG-44细胞，同时设立空白对照组和阴性对照组，实时荧光定量PCR检测转染前后miR-195的表达水平；用CCK-8法评价细胞的增殖能力；流式细胞仪分析细胞周期。结果胶质瘤细胞转染miR-195 mimics后，发现U251细胞和SHG-44细胞中miR-195的表达分别为5.93±0.43和6.43±0.48，较空白对照组和阴性对照组均增高约6倍左右。与空白对照组和阴性对照组相比，转染miR-195 mimics后的胶质瘤细胞的增殖能力明显受到抑制，细胞周期阻滞于G0/G1期，S期细胞减少。结论过表达miR-195可以阻滞胶质瘤细胞周期进展，从而抑制其增殖，为胶质瘤的基因治疗提供新的策略。
목적탐토 miR-195과표체대체외배양적인뇌효질류세포U251화 SHG-44증식적영향。방법통과지질체장miR-195 mimics전염지U251화SHG-44세포，동시설립공백대조조화음성대조조，실시형광정량PCR검측전염전후miR-195적표체수평；용CCK-8법평개세포적증식능력；류식세포의분석세포주기。결과효질류세포전염miR-195 mimics후，발현U251세포화SHG-44세포중miR-195적표체분별위5.93±0.43화6.43±0.48，교공백대조조화음성대조조균증고약6배좌우。여공백대조조화음성대조조상비，전염miR-195 mimics후적효질류세포적증식능력명현수도억제，세포주기조체우G0/G1기，S기세포감소。결론과표체miR-195가이조체효질류세포주기진전，종이억제기증식，위효질류적기인치료제공신적책략。
Objective To explore the effect of miR-195 on proliferation of U251 and SHG-44 glioblastoma cells.Methods miR-195 mimics were transfected into U251 and SHG-44 cells by Lipofectamine RNAiMAX .At the same time, the blank control group and negative control group were established .miR-195 expression level was identified by quantitative real-time polymerase chain reaction ( qRT-PCR ) .The ability of cell proliferation was detected by CCK8.Using flow cytometry to test and analyse cell cycle between the different groups .Results After transfection of miR-195 mimics, Real-time PCR showed that miR-195 expression level increased significantly . Compared with the blank control group and negative control group , after over-expression of miR-195 , glioma cells proliferation was obviously inhibited .By flow cytometry on cell cycle analysis , results showed that cell cycle was arrested in G0/G1 phase and S phase cells were decreased .Conclusion Over-expression of miR-195 could inhibit proliferation,block G1/S transition.miR-195 may play a potential role in glioma gene therapy .