CAJ | 학술논문

目的对直肠癌miRNA表达谱进行初步研究，以期发现在直肠癌发生过程中与直肠腺瘤miRNA的差异表达谱，并预测可能受其调控的靶基因。方法选择2011年1～3月于解放军第306医院肠镜检查的直肠癌及直肠腺瘤进行检测，筛选直肠癌、直肠腺瘤组织差异表达的miRNA，并运用生物信息软件分析其靶基因。结果通过miRNAs芯片检测，发现直肠腺癌与直肠腺瘤相比，上调的miRNA 34种，下调8种。预测ZFHX4可能为miR-375的靶基因，预测SMAD2等可能为miR-18a的靶基因。结论与直肠腺瘤相比，直肠腺癌有其特异的miRNA表达谱，miRNA在直肠癌的发生中可能起到重要作用。
목적대직장암miRNA표체보진행초보연구，이기발현재직장암발생과정중여직장선류miRNA적차이표체보，병예측가능수기조공적파기인。방법선택2011년1～3월우해방군제306의원장경검사적직장암급직장선류진행검측，사선직장암、직장선류조직차이표체적miRNA，병운용생물신식연건분석기파기인。결과통과miRNAs심편검측，발현직장선암여직장선류상비，상조적miRNA 34충，하조8충。예측ZFHX4가능위miR-375적파기인，예측SMAD2등가능위miR-18a적파기인。결론여직장선류상비，직장선암유기특이적miRNA표체보，miRNA재직장암적발생중가능기도중요작용。
Objective The miRNA expression profiles in rectal cancer and rectal adenoma are not examined till now in our country .The objective of this study is to compare and contrast the expression of miRNAs between rectal adenoma and adenocarcinoma ,and to identify some of the genes possibly associated with the development of rectal cancer.Methods We studied two rectal adenocarcinomas and two rectal adenomas samples to examine miRNA expression profiles through miRNA microarray .We also predicted the possible targets of miRNA by bioinformatics . Results Forty-two miRNAs were found to be differentially expressed between rectal adenocarcinomas and adenomas tissues,with thirty-four miRNAs upregulated while eight downregulated .Bioinformatics indicated that ZFHX 4 may be the targets of miR-375,and that SMAD2 may be the target of miR-18a.Conclusion There is a specific miRNA profile in rectal adenocarcinoma comparing to rectal adenoma .miRNA pathways play an important role in genes and have development in rectal adenocarcinoma .