山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2014年
16期
7-9
,共3页
单侧输尿管梗阻%超声造影%血流灌注%肾髓质%大鼠
單側輸尿管梗阻%超聲造影%血流灌註%腎髓質%大鼠
단측수뇨관경조%초성조영%혈류관주%신수질%대서
contrast-enhanced ultrasound%unilateral ureteral obstruction%blood perfusion%kidney medulla%rat
目的：采用超声造影（ CEUS）定量技术评估单侧输尿管梗阻（ UUO）大鼠肾髓质血流灌注的变化。方法建立大鼠UUO模型19只，采用CEUS及定量分析技术检测造模前后双侧肾髓质血流灌注，并行病理检查。结果造模后梗阻侧肾髓质较造模前血流灌注速度减慢，造影剂廓清时间延长，但增强程度变化不大；健侧肾髓质较造模前血流灌注速度、强度及造影剂廓清时间变化不大。造模前后梗阻侧肾髓质血流灌注时间-强度曲线中达峰时间（TTP）分别为（25．59±3．92）、（28．48±4．67）s，曲线下面积（AUC）分别为（905．46±271．90）、（1010．54±309．11）dB· s，曲线上升支斜率（A）分别为（1．04±0．59）、（0．87±0．41）dB/s，P均＜0．05。造模前后健侧肾髓质TTP、AUC、A、峰值强度（PI）比较， P均＞0．05。梗阻侧肾脏近曲小管及远曲小管均出现不同程度的扩张，肾间质水肿增宽，并出现单核巨噬细胞、淋巴细胞的浸润，偶见肾小管萎缩。结论 UUO大鼠梗阻侧肾髓质局部组织的血流灌注速度降低，血管内血流容积增大；CEUS及定量分析技术能较好地检测UUO大鼠肾髓质血流灌注的变化。
目的：採用超聲造影（ CEUS）定量技術評估單側輸尿管梗阻（ UUO）大鼠腎髓質血流灌註的變化。方法建立大鼠UUO模型19隻，採用CEUS及定量分析技術檢測造模前後雙側腎髓質血流灌註，併行病理檢查。結果造模後梗阻側腎髓質較造模前血流灌註速度減慢，造影劑廓清時間延長，但增彊程度變化不大；健側腎髓質較造模前血流灌註速度、彊度及造影劑廓清時間變化不大。造模前後梗阻側腎髓質血流灌註時間-彊度麯線中達峰時間（TTP）分彆為（25．59±3．92）、（28．48±4．67）s，麯線下麵積（AUC）分彆為（905．46±271．90）、（1010．54±309．11）dB· s，麯線上升支斜率（A）分彆為（1．04±0．59）、（0．87±0．41）dB/s，P均＜0．05。造模前後健側腎髓質TTP、AUC、A、峰值彊度（PI）比較， P均＞0．05。梗阻側腎髒近麯小管及遠麯小管均齣現不同程度的擴張，腎間質水腫增寬，併齣現單覈巨噬細胞、淋巴細胞的浸潤，偶見腎小管萎縮。結論 UUO大鼠梗阻側腎髓質跼部組織的血流灌註速度降低，血管內血流容積增大；CEUS及定量分析技術能較好地檢測UUO大鼠腎髓質血流灌註的變化。
목적：채용초성조영（ CEUS）정량기술평고단측수뇨관경조（ UUO）대서신수질혈류관주적변화。방법건립대서UUO모형19지，채용CEUS급정량분석기술검측조모전후쌍측신수질혈류관주，병행병리검사。결과조모후경조측신수질교조모전혈류관주속도감만，조영제곽청시간연장，단증강정도변화불대；건측신수질교조모전혈류관주속도、강도급조영제곽청시간변화불대。조모전후경조측신수질혈류관주시간-강도곡선중체봉시간（TTP）분별위（25．59±3．92）、（28．48±4．67）s，곡선하면적（AUC）분별위（905．46±271．90）、（1010．54±309．11）dB· s，곡선상승지사솔（A）분별위（1．04±0．59）、（0．87±0．41）dB/s，P균＜0．05。조모전후건측신수질TTP、AUC、A、봉치강도（PI）비교， P균＞0．05。경조측신장근곡소관급원곡소관균출현불동정도적확장，신간질수종증관，병출현단핵거서세포、림파세포적침윤，우견신소관위축。결론 UUO대서경조측신수질국부조직적혈류관주속도강저，혈관내혈류용적증대；CEUS급정량분석기술능교호지검측UUO대서신수질혈류관주적변화。
Objective To evaluate the renal medulla blood perfusion characteristics in rats with unilateral ureteral ob -struction (UUO) by using contrast-enhanced ultrasound (CEUS) and quantitative analysis technique .Methods Nineteen UUO rat models were established , and the bilateral renal medullary perfusion before and after the UUO operation was detec -ted by using CEUS and quantitative analysis technique , meanwhile , pathology examination was performed .Results After modeling , the blood perfusion velocity of the obstructed kidney medulla was slower than before modeling , the contrast agent washout time was prolonged , but the enhancement degree changed little;while, the blood perfusion velocity , intensity and contrast agent washout time changed little before and after the modeling of the normal renal medulla .Quantitative analysis showed that the preoperative and postoperative blood perfusion time to peak ( TTP) in the obstructed kidney medulla were (25.59 ±3.92) and (28.48 ±4.67)s, area under curve (AUC) were (905.46 ±271.90) and (1 010.54 ±309.11) dB · s, and slope rate of ascending curve (A) were (1.04 ±0.59) and (0.87 ±0.41)dB/s, respectively (all P<0.05). No significantly differences were found in TTP, AUC, A and PI of the normal renal medulla (P>0.05).The obstructed renal proximal tubule and distal convoluted tubules showed varying degrees of expansion , broadening renal interstitial ede-ma, and infiltration of mononuclear macrophage and lymphocyte , occasionally tubular atrophy .Conclusions The obstruc-ted renal medulla blood perfusion velocity of UUO rats decreases and intravascular blood volume increases .CEUS and quantitative analysis technique may effectively detect the changes of renal medulla perfusion in UUO rats .
