CAJ | 학술논문

为便于猪多杀性巴氏杆菌病活疫苗（ EO630株）质量控制和降低生产成本，研制了ZJ-1和ZJ-2两种合成培养基，并进行了两种合成培养基与马丁肉汤的比较试验。结果表明，合成培养基ZJ-1培养活菌数高于ZJ-2和马丁肉汤。用合成培养基ZJ-1培养制备3批疫苗，并分别用兔进行安全检验和小鼠效力检验。3批疫苗对兔均2／2健活，对小鼠的保护力分别为9／10、9／10、8／10，符合产品质量标准。本试验表明，运用合成培养基替代传统培养基是可行的。
위편우저다살성파씨간균병활역묘（ EO630주）질량공제화강저생산성본，연제료ZJ-1화ZJ-2량충합성배양기，병진행료량충합성배양기여마정육탕적비교시험。결과표명，합성배양기ZJ-1배양활균수고우ZJ-2화마정육탕。용합성배양기ZJ-1배양제비3비역묘，병분별용토진행안전검험화소서효력검험。3비역묘대토균2／2건활，대소서적보호력분별위9／10、9／10、8／10，부합산품질량표준。본시험표명，운용합성배양기체대전통배양기시가행적。
To facilitate the production of swine Pasteurella multocida vaccine,live( strain EO630) and decrease the manufacturing cost of the culture media, we have developed such two synthetic mediums as ZJ-1and ZJ-2 and made the contrast experiment with Martin broth for effects. The results showed that the amounts of live bacteria prepared by ZJ-1 were significantly higher than those prepared by ZJ-2 and Martin broth. Using ZJ-1 to produce swine Pasteurella multocida vaccine,live( strain EO630) , We have got the products in three lots and made related security detection and potency test. In consequence, the results showed that the survival rate of two rabbits were all 100%( 2/2 ) and the protective efficacy for mice were 90%( 9/10 ) , 90%( 9/10 ) and 80%( 8/10 ) respectively, which met the standard requirements of the quality of products. The experiment suggested that it was feasible to replace traditional culture medium with synthetic medium.