CAJ | 학술논문

目的通过对大肠癌组织中P-gp表达情况的检测，分析P-gp表达与大肠癌临床病理参数的关系，探讨利用其评估大肠癌多药耐药性的可能性，希望能为大肠癌综合化疗方案的制定提供一些帮助。㎡方法㎡本研究收集大连大学附属新华医院自2011年1月至2011年12月间经手术切除的大肠癌标本306例，应用免疫组化方法，检测此306例大肠癌组织中P-gp表达情况，同时将Ki67蛋白作为细胞增殖的标志一并检测其表达情况；分析P-gp表达情况与患者的性别、年龄以及肿瘤的生长部位、分化程度、肠壁浸润深度、淋巴结转移情况、Ki67蛋白表达之间的关系。㎡结果㎡ P-gp表达阳性率在本组大肠癌组织中为77.12%。其表达与肿瘤浸润深度呈显著正相关关系(P=0.038＜0.05)；与淋巴结转移情况二者之间差异显著(P=0.022＜0.05)；与Ki67蛋白表达二组之间差异显著(P=0.007＜0.05)。然而， P-gp的表达与患者的性别、年龄及肿瘤的生长部位、分化程度之间均无显著相关关系(P＞0.05)。㎡结论㎡通过对大肠癌组织中P-gp表达情况的检测，可以对大肠癌的多药耐药性做出评估，对化疗方案的制定提供帮助。
목적통과대대장암조직중P-gp표체정황적검측，분석P-gp표체여대장암림상병리삼수적관계，탐토이용기평고대장암다약내약성적가능성，희망능위대장암종합화료방안적제정제공일사방조。㎡방법㎡본연구수집대련대학부속신화의원자2011년1월지2011년12월간경수술절제적대장암표본306례，응용면역조화방법，검측차306례대장암조직중P-gp표체정황，동시장Ki67단백작위세포증식적표지일병검측기표체정황；분석P-gp표체정황여환자적성별、년령이급종류적생장부위、분화정도、장벽침윤심도、림파결전이정황、Ki67단백표체지간적관계。㎡결과㎡ P-gp표체양성솔재본조대장암조직중위77.12%。기표체여종류침윤심도정현저정상관관계(P=0.038＜0.05)；여림파결전이정황이자지간차이현저(P=0.022＜0.05)；여Ki67단백표체이조지간차이현저(P=0.007＜0.05)。연이， P-gp적표체여환자적성별、년령급종류적생장부위、분화정도지간균무현저상관관계(P＞0.05)。㎡결론㎡통과대대장암조직중P-gp표체정황적검측，가이대대장암적다약내약성주출평고，대화료방안적제정제공방조。
Objective We detected the expression of P-gp in colorectal cancer and analyzed the correlation of the protein expression to the clinical pathological parameters of the patients with colorectal cancer, explored the feasibility to assess of multidrug resistance of colorectal cancer with P-gp, it maybe help us to decide the chemotherapy program. Methods] Three hundred and six surgical resection cases of colorectal cancer were selected from January 2011 to December 2011 in Dalian University Affiliated Xinhua Hospital. The expressionof P-gp in the tumor tissues were detected by immunohistochemistry. The expression of Ki67 protein was also detected and then used as the marker for cell proliferation. We analyzed the correlations of the expression of P-gp to the patients' gender, age and tumor's growth position, differentiation, infiltrating depth, lymph node metastases and the expression of Ki67 protein. Results] The positive rate of P-gp expression in colorectal cancer was 77.12%.The expression of P-gp was positively significant correlation to infiltrating depth of the tumor (P=0.038<0.05); there was significant difference with the lymph node metastases of tumors (P=0.022<0.05);and there was significant difference with the expression of Ki67 protein (P=0.007<0.05); but the expression of P-gp had no significant correlation to patients' gender, age and tumor's site, differentiation (P>0.05).Conclusion]By detecting the expression of P-gp in colorectal cancer, we could assess the multidrug resistance of colorectal cancer, it can help us to decide the chemotherapy program.