CAJ | 학술논문

以硫酸软骨素的吸附率和解析率为指标，通过静态吸附实验筛选出D290阴离子交换树脂用于高效分离酶解液中的硫酸软骨素，考察了平衡时间、初始浓度、pH、温度等因素对鲨鱼硫酸软骨素分离效果的影响。较佳的分离条件如下：酶解液的初始浓度20 mg/mL，pH 5，室温，静态吸附9 h，洗脱溶剂3 mol/L NaCl溶液。在该条件下，硫酸软骨素的静态饱和吸附量（以湿树脂计）为98.9 mg/g，解析率为87.2%，硫酸软骨素产品的纯度为96.5%。D290阴离子交换树脂具有选择性好、吸附率和解析率高等优点，适用硫酸软骨素的分离纯化。
이류산연골소적흡부솔화해석솔위지표，통과정태흡부실험사선출D290음리자교환수지용우고효분리매해액중적류산연골소，고찰료평형시간、초시농도、pH、온도등인소대사어류산연골소분리효과적영향。교가적분리조건여하：매해액적초시농도20 mg/mL，pH 5，실온，정태흡부9 h，세탈용제3 mol/L NaCl용액。재해조건하，류산연골소적정태포화흡부량（이습수지계）위98.9 mg/g，해석솔위87.2%，류산연골소산품적순도위96.5%。D290음리자교환수지구유선택성호、흡부솔화해석솔고등우점，괄용류산연골소적분리순화。
The static chondroitin sulfate adsorption and desorption ratio were used as parameters to select the resin. D290 anion-exchange resin was the best resin for separation of chondroitin sulfate from shark cartilage hydrolysates. The effects of equilibrium time, initial concentration, pH and temperature on the adsorption were investigated. The optimum separation condition was as follows:equilibrium time 9 h, initial chondroitin sulfate concentration 20.0 mg/mL, pH 5 and 25℃. The elution solvent was 3 mol/L NaCl solution. Under the condition, the static saturated adsorption capacity of chondroitin sulfate (by wet resin) was 98.9 mg/g, desorption ratio was 87.2%, and the purity of chondroitin sulfate product was 96.5%. D290 anion-exchange resin was suitable for separation of chondroitin sulfate with high adsorption and desorption ratio.