CAJ | 학술논문

目的观察雄性大鼠缺氧后头颈部真皮雌激素受体mRNA表达的变化。方法成年雄性Wistar大鼠置于缺氧舱中，每天缺氧处理4 h ，分别缺氧处理1、5、10、15、20、30 d。采用逆转录-聚合酶链式反应（RT-PCR），检测面部真皮雌激素受体（ER）α、βmRNA表达。结果缺氧处理后，ERβmRNA的表达则表现为缺氧后1天略有升高，但差异无统计学意义，5 d后ERβmRNA表达明显升高（P＜0．05），10 d后 ERβmRNA表达有所回落；15、20 d后ERβmRNA表达再次升高，其中20 d的表达与对照组比较差异有统计学意义（ P＜0．01）。缺氧30 d 后ERβmRNA表达再次下降至对照组水平。结论缺氧可在转录水平上影响大鼠面部真皮ERβmRNA表达。
목적관찰웅성대서결양후두경부진피자격소수체mRNA표체적변화。방법성년웅성Wistar대서치우결양창중，매천결양처리4 h ，분별결양처리1、5、10、15、20、30 d。채용역전록-취합매련식반응（RT-PCR），검측면부진피자격소수체（ER）α、βmRNA표체。결과결양처리후，ERβmRNA적표체칙표현위결양후1천략유승고，단차이무통계학의의，5 d후ERβmRNA표체명현승고（P＜0．05），10 d후 ERβmRNA표체유소회락；15、20 d후ERβmRNA표체재차승고，기중20 d적표체여대조조비교차이유통계학의의（ P＜0．01）。결양30 d 후ERβmRNA표체재차하강지대조조수평。결론결양가재전록수평상영향대서면부진피ERβmRNA표체。
Objective To observe the expression of estrogen receptor (ER) mRNA in male rat facial skin ex-posed to hypoxia .Methods Adult male Wistar rats were exposed to hypoxia module for 4 h everyday for 1 ,5 ,10 , 15 ,20 and 30 days .Levels of ERαand ERβmRNA in facial skin tissue were measured by using semi-quantitative re-al-time polymerase chain reaction .Results The expression of ERβmRNA increased since the 1st day ,but was not significant .While ,after the 5th day ,the expression level significantly increased (P<0 .05) .Level of ERβmRNA began to decrease after 10 days ,but increased again after 15 and 20 days of treatment ,and was significantly higher than control group after 20 days (P<0 .01) .After treatment of 30 days ,level of ERβmRNA were decreased again . Conclusion Hypoxia might affect the transcription of ERβin male adult rat facial skin .