CAJ | 학술논문

目的探讨胰腺癌组织中表皮生长因子受体（EGFR）和肝细胞生长因子受体（Met）基因和蛋白的表达情况，及其与胰腺癌临床病理特征和预后的关系。方法采用免疫组化法检测78例胰腺癌组织和23例正常胰腺组织中EGFR和Met蛋白的表达情况，采用实时定量聚合酶链反应（RT-RCR）法检测 EGFR和Met-DNA的相对拷贝数。结果78例胰腺癌组织中，EGFR和Met蛋白的阳性表达率明显高于正常胰腺组织，差异均有统计学意义（ P＜0．05）。肿瘤直径大于4 cm患者、胰腺癌组织高中分化患者、出现远处转移患者、淋巴转移患者、肠系膜上血管侵犯患者、T N M分期为Ⅲ和Ⅳ期患者中EG FR和M et蛋白的表达均明显高于肿瘤直径小于或等于4 cm患者，胰腺癌组织低分化患者，未出现远处转移患者、未有淋巴转移患者、未出现肠系膜上血管侵犯患者、TNM 分期为Ⅰ和Ⅱ期患者，差异具有统计学意义（P＜0．05）。胰腺癌患者中，EGFR和Met-DNA 相对拷贝数均明显高于正常胰腺组织中EGFR和Met-DNA相对拷贝数，差异具有统计学意义（P＜0．05）。高表达EGFR和Met胰腺癌患者的平均生存时间均显著低于Met及EGFR低表达患者，差异具有统计学意义（P＜0．05）。Met和EGFR均高表达的患者的平均生存时间较Met或 EGFR单一高表达者的短，差异具有统计学意义（P＜0．05）。结论 EGFR和M et的表达与胰腺癌的临床病理相关指标密切相关，对二者的分析研究有助于对胰腺癌患者的预后作出预测，并对化疗药物新靶点筛选提供理论依据。
목적탐토이선암조직중표피생장인자수체（EGFR）화간세포생장인자수체（Met）기인화단백적표체정황，급기여이선암림상병리특정화예후적관계。방법채용면역조화법검측78례이선암조직화23례정상이선조직중EGFR화Met단백적표체정황，채용실시정량취합매련반응（RT-RCR）법검측 EGFR화Met-DNA적상대고패수。결과78례이선암조직중，EGFR화Met단백적양성표체솔명현고우정상이선조직，차이균유통계학의의（ P＜0．05）。종류직경대우4 cm환자、이선암조직고중분화환자、출현원처전이환자、림파전이환자、장계막상혈관침범환자、T N M분기위Ⅲ화Ⅳ기환자중EG FR화M et단백적표체균명현고우종류직경소우혹등우4 cm환자，이선암조직저분화환자，미출현원처전이환자、미유림파전이환자、미출현장계막상혈관침범환자、TNM 분기위Ⅰ화Ⅱ기환자，차이구유통계학의의（P＜0．05）。이선암환자중，EGFR화Met-DNA 상대고패수균명현고우정상이선조직중EGFR화Met-DNA상대고패수，차이구유통계학의의（P＜0．05）。고표체EGFR화Met이선암환자적평균생존시간균현저저우Met급EGFR저표체환자，차이구유통계학의의（P＜0．05）。Met화EGFR균고표체적환자적평균생존시간교Met혹 EGFR단일고표체자적단，차이구유통계학의의（P＜0．05）。결론 EGFR화M et적표체여이선암적림상병리상관지표밀절상관，대이자적분석연구유조우대이선암환자적예후작출예측，병대화료약물신파점사선제공이론의거。
Objective To study the clinical significance of hepatocyte growth factor receptor (Met) and epider-mal growth factor receptor(EGFR) in pancreatic cancer .Methods Expression levels of EGFR and Met protein were detected by immunohistochemical staining in 78 cases of pancreatic cancer tissues and 23 cases of normal pancreatic tissues ,and gene copy numbers of EGFR and Met were detected by fluorescent real time-polymerase chain reaction . Results Positive rates of EGFR and Met expression in pancreatic cancer tissues were higher than those in normal tis-sues (P<0 .05) .Expression levels of EGFR and Met were significantly influenced by TNM stages ,tumor size ,su-perior mesenteric vessels invasion and transfer of tumor lesions (P<0 .05) .Relative gene copy numbers of EGFR and Met in pancreatic cancer tissues were significantly higher than those in normal pancreatic tissues (P<0 .05) . The median survival time of pancreatic cancer patients with high expression of EGFR or Met were significantly lower than patients with low expression of Met or EGFR (P<0 .05) .The median survival time of patients with high ex-pression of Met and EGFR were significantly lower than patients with high expression of Met or EGFR (P<0 .05) . Conclusion EGFR and Met might interact in a synergistic manner with the oncogenesis and development of pancre-atic cancer ,could be helpful for the prognostic evaluation of pancreatic cancer and also could be new therapeutic tar-gets in pancreatic cancer .