CAJ | 학술논문

以愈创木酚培养基为底物，利用平板筛选法从土壤朽木中筛选能够产漆酶的菌株，通过测定漆酶活力进行复筛，筛选出一株产漆酶活力较高的白腐菌菌株。以漆酶活性为参考指标，确定葵花粉发酵产漆酶条件：发酵培养基中硫酸铵0．025％，初始pH值5．5，接种量8％，装液量50mL，愈创木酚0．10％，培养时间7d。
이유창목분배양기위저물，이용평판사선법종토양후목중사선능구산칠매적균주，통과측정칠매활력진행복사，사선출일주산칠매활력교고적백부균균주。이칠매활성위삼고지표，학정규화분발효산칠매조건：발효배양기중류산안0．025％，초시pH치5．5，접충량8％，장액량50mL，유창목분0．10％，배양시간7d。
To guaiacol as the substrate ,a laccase-producing strain was screened by medium flats and rescreened by measuring the laccase activity from soil and rotten wood samples .A high yield white-rot fungus for laccase produc-tion was screened .As a reference index to determining the laccase activity , a better culturing condition was ob-tained.The results were ammonium sulfate 0.025%,culture initial pH5.5,inoculum 8%,250 mL flask with 50mL medium,guaiacol 0.10%and the culture time 7d.