CAJ | 학술논문

目的：研究虎金丸对肝纤维化大鼠Nrf2/Bach1蛋白表达的影响，初步探讨虎金丸调整机体氧化应激抗肝纤维化的作用机制。方法以CCl4诱导肝纤维化大鼠动物模型，采用Masson胶原染色肝脏纤维化，分光光度法检测SOD、GSH-Px含量，免疫组化法测检Nrf2、Bach1的蛋白量。结果从Masson染色得出，虎金丸能减轻CCL4所致的肝纤维化，显著降低胶原纤维面密度；与模型组比较，虎金丸组SOD、GSH等抗氧化酶的含量显著升高（ P＜0.01)；虎金丸药物的使用显著降低了肝细胞组织匀浆中Nrf2的表达水平、提高了Bach1的表达水平（P＜0.01)。结论虎金丸可调控肝细胞组织匀浆中Nrf2/Bach1蛋白的表达，促进机体氧化应激的调整与抗肝纤维化。
목적：연구호금환대간섬유화대서Nrf2/Bach1단백표체적영향，초보탐토호금환조정궤체양화응격항간섬유화적작용궤제。방법이CCl4유도간섬유화대서동물모형，채용Masson효원염색간장섬유화，분광광도법검측SOD、GSH-Px함량，면역조화법측검Nrf2、Bach1적단백량。결과종Masson염색득출，호금환능감경CCL4소치적간섬유화，현저강저효원섬유면밀도；여모형조비교，호금환조SOD、GSH등항양화매적함량현저승고（ P＜0.01)；호금환약물적사용현저강저료간세포조직균장중Nrf2적표체수평、제고료Bach1적표체수평（P＜0.01)。결론호금환가조공간세포조직균장중Nrf2/Bach1단백적표체，촉진궤체양화응격적조정여항간섬유화。
Objective To investigate the effects of Hujin pills on the expression of Nrf2/Bach1 proteins of rats with liver fibrosis,and to explore the mechanism of Hujin pills in regulating oxidative stress for anti-hepatic fibrosis. Methods The rat models with liver fibrosis were induced by CCl4.Masson collagen was used to stain the liver fibrosis.SOD and GSH-Px were measured by spectrophotometry.Nrf2 and Bach1 proteins were measured by immunohistochemistry. Results The masson staining experiment shows that Hujin pill could significantly reduce the collagen fibers surface density(P<0.01) therefore alleviate the liver fibrosis induced by CCL4.Compared with model group,the Hujin pill group saw an significant increase in the Content of SOD, GSH and other antioxidant enzymes in the liver homogenate(P<0.01); The administration of Hujin Pills contributed to a decline of Nrf2 expression and an increase of Bach1 expression level in liver cell cytoplasm. Conclusion Hujin pills can adjust the level and expression of Nrf2/Bach1 proteins in the liver homogenate as well as improve the adjustment of oxidative stress and anti-hepatic fibrosis.