CAJ | 학술논문

目的：探讨环介导等温扩增技术用于检测临床标本乳头瘤病毒HPV18亚型的应用价值。方法设计针对HPV18-L1基因序列中6个区段的4条特异引物，在等温条件下（65℃）进行核酸扩增反应1h，在扩增前加入钙黄绿素作为反应指示剂；利用建立的环介导等温扩增方法进行敏感型检测以及检测正常体检妇女和患者子宫颈分泌物的HPV18亚型。结果 LAMP检测HPV18 L1基因的敏感度为1000拷贝每微升；阴道炎患者HPV18阳性检出率为5.26%（1/19）；慢性子宫颈炎组HPV18亚型检出率为10%（2/20）；宫颈上皮内瘤变（CINⅠ~Ⅱ级）阳性检出率33.3%（3/9）；子宫颈癌Ⅰ级阳性检出率为64％（16/25）。结论基于目视的环介导等温扩增方法是一种具有经济、快速筛查临床标本中HPV18亚型的诊断工具，可具有在基层医院推广应用的潜能。
목적：탐토배개도등온확증기술용우검측림상표본유두류병독HPV18아형적응용개치。방법설계침대HPV18-L1기인서렬중6개구단적4조특이인물，재등온조건하（65℃）진행핵산확증반응1h，재확증전가입개황록소작위반응지시제；이용건립적배개도등온확증방법진행민감형검측이급검측정상체검부녀화환자자궁경분비물적HPV18아형。결과 LAMP검측HPV18 L1기인적민감도위1000고패매미승；음도염환자HPV18양성검출솔위5.26%（1/19）；만성자궁경염조HPV18아형검출솔위10%（2/20）；궁경상피내류변（CINⅠ~Ⅱ급）양성검출솔33.3%（3/9）；자궁경암Ⅰ급양성검출솔위64％（16/25）。결론기우목시적배개도등온확증방법시일충구유경제、쾌속사사림상표본중HPV18아형적진단공구，가구유재기층의원추엄응용적잠능。
Objective To investigate the application of loop-mediated isothermal amplification(LAMP)in detecting Human papillomavirus type 18(HPV18) from clinical specimens. Methods It is characterized by employing a set of four specially designed primers spanning six distinct sequences of a target gene HPV18-L1. The reaction was performed at 65℃for 1 hour with the addition of calcein dye prior to amplification. LAMP was used for sensitivity assay and detecting HPV18 mono infected in cervical secretions from women of physical examination and patients. Results The sensitivity of LAMP for detection of HPV18 L1 is 10-3 per microliter;the positive rate of HPV18 in patients with vaginitis is 5.26%(1/19); the positive rate of HPV18 in patients with chronic cervicitis is 10%(2/20); and the positive rate of HPV18 in patients with cervical intraepithelial neoplasia (CIN I-Ⅱ)and cervical cancer-I is 33.3%(3/9), 64%(16/25), respectively. Conclusion LAMP assay is a high efficiency, low cost diagnostic tool, useful for rapid, accurate, direct detection of HPV for clinical diagnosis and might be popular at the primary hospital in future.