中医临床研究
中醫臨床研究
중의림상연구
CLINICAL JOURNAL OF CHINESE MEDICINE
2013年
19期
27-28
,共2页
车彦忠%王培智%陈洪英%袁卫斌
車彥忠%王培智%陳洪英%袁衛斌
차언충%왕배지%진홍영%원위빈
护肝宁丸%虎杖苷%HPLC法
護肝寧汍%虎杖苷%HPLC法
호간저환%호장감%HPLC법
Huganning pills%Polydatin%HPLC
目的：建立复方制剂护肝宁丸中的有效成分虎杖苷的含量测定方法。方法：HPLC法。色谱条件：色谱柱ZORBAX SB-C18（4.6×250nm 5μm），以乙腈-水（20∶80）为流动相；检测波长：306nm；流速：1.0ml/min。结果：虎杖苷在0.1692～1.269μg的范围内与峰面积呈良好的线性关系，r＝0.9996，平均回收率为98.29℅，RSD为0.79℅。结论：本测定方法简便可行，重复性好，可用于本制剂中有效成分虎杖苷的含量测定。
目的：建立複方製劑護肝寧汍中的有效成分虎杖苷的含量測定方法。方法：HPLC法。色譜條件：色譜柱ZORBAX SB-C18（4.6×250nm 5μm），以乙腈-水（20∶80）為流動相；檢測波長：306nm；流速：1.0ml/min。結果：虎杖苷在0.1692～1.269μg的範圍內與峰麵積呈良好的線性關繫，r＝0.9996，平均迴收率為98.29℅，RSD為0.79℅。結論：本測定方法簡便可行，重複性好，可用于本製劑中有效成分虎杖苷的含量測定。
목적：건립복방제제호간저환중적유효성분호장감적함량측정방법。방법：HPLC법。색보조건：색보주ZORBAX SB-C18（4.6×250nm 5μm），이을정-수（20∶80）위류동상；검측파장：306nm；류속：1.0ml/min。결과：호장감재0.1692～1.269μg적범위내여봉면적정량호적선성관계，r＝0.9996，평균회수솔위98.29℅，RSD위0.79℅。결론：본측정방법간편가행，중복성호，가용우본제제중유효성분호장감적함량측정。
Objective: To determine the concents of baicalin in polydatin in Huganning Pills. Methods: The HPLC method was established for the determination of polydatin in Huganning pills. The column is ZORBAX SB-C18 (4.6×250mm 5μm), mobile phase consisting of acetonitrile-water (20∶80), and the flow rare was 1.0ml/min, the detection wavelength is 306nm. Results: A better Liner relation was obtained form 0.1692 to 1.269μg of polydatin for plasma with a good correlation coefficient;r=0.9996, the average recovery of ephedrine hydrochloride was 98.29℅, RSD was 0.79℅, the method has good precision repeatability. Conclusion:This method is simple, and quick.it can be used as quality control in Huganning pills.
