CAJ | 학술논문

目的观察心肌细胞间粘附分子-1（ICAM-1）在脓毒症大鼠心肌细胞的表达，同时探讨辛伐他汀预处理对其表达的影响及意义。方法雌性Wistar大鼠60只随机分为正常组、脓毒症组和辛伐他汀组，每组20例，应用盲肠结扎穿孔法制造脓毒症模型。辛伐他汀组喂养辛伐他汀20 mg/kg，1次/d，共2周。2周后辛伐他汀组与脓毒症组大鼠行盲肠结扎穿孔术。术后48 h取各组大鼠心肌组织制成石蜡切片，采用免疫组织化学染色方法观察心肌细胞ICAM-1的蛋白表达。结果免疫组织化学染色显示脓毒症组大鼠心肌细胞ICAM-1蛋白表达于术后48 h较正常组明显增加（ P ＜0铑．05），辛伐他汀组大鼠心肌细胞ICAM-1蛋白表达与脓毒症组比较明显减弱（ P ＜0．05），与正常组比较差异有统计学意义（ P ＜0．05）。
목적관찰심기세포간점부분자-1（ICAM-1）재농독증대서심기세포적표체，동시탐토신벌타정예처리대기표체적영향급의의。방법자성Wistar대서60지수궤분위정상조、농독증조화신벌타정조，매조20례，응용맹장결찰천공법제조농독증모형。신벌타정조위양신벌타정20 mg/kg，1차/d，공2주。2주후신벌타정조여농독증조대서행맹장결찰천공술。술후48 h취각조대서심기조직제성석사절편，채용면역조직화학염색방법관찰심기세포ICAM-1적단백표체。결과면역조직화학염색현시농독증조대서심기세포ICAM-1단백표체우술후48 h교정상조명현증가（ P ＜0로．05），신벌타정조대서심기세포ICAM-1단백표체여농독증조비교명현감약（ P ＜0．05），여정상조비교차이유통계학의의（ P ＜0．05）。
Objective To observe the expressions of ICAM-1 in myocardial cells of rats with sepsis ,and to explore the effect and significance of simvastatin pretreatment on ICAM-1 expression .Methods Sixty female Wistar rats were randomly divided into three groups:control group ( n =20 ) , sepsis group ( n =20 ) and simvastatin group ( n =20 ) .The animal models with sepsis were established by cecal ligation and puncture (CLP).The rats in simvastatin group were treated with simvastatin ,20mg/kg,once a day for two weeks before CLP.After teo weeks the rats both simvastatin group and sepsis group underwent CLP ,then 48h after operation the myocardium tissues of rats in three groups were collected to be made paraffin section , and the expressions of ICAM-1 protein in myocardial cells were detected by immunohistochemistry ( IH ) staining.Results Immunohistochemistry staining showed that the expression levels of ICAM-1 in sepsis group were significantly higher than those in control group at 48h after CLP ( P <0.05),however,which in simvastatin group were significantly decreased,as ompared with those in sepsis group ( P <0.05),and there were significant differences in expression levels of ICAM-1 between simvastatin group and control group ( P <0.05 ).Conclusion Simvastatin can attenuate the expression of ICAM-1 in myocardial cells of rats with sepsis ,as a result,which can partially improve heart function during sepsis .