CAJ | 학술논문

目的研究人舌癌组织中环氧化酶-2（ Cox-2）、β-连环蛋白（β-catenin ）及Survivin在基因水平的表达及相互关系，为舌癌的诊断、治疗及预后提供参考依据。方法应用逆转录-聚合酶链反应（ RT-PCR）方法检测Cox-2 mRNA、β-catenin mRNA及Survivin mRNA在60例舌癌和30例正常口腔黏膜中的表达情况，分析它们在舌癌组织中表达的差异性和相关性。结果 Cox-2 mRNA和Survivin mRNA在舌癌组织中呈强阳性表达，而在正常口腔黏膜均为阴性表达，差异有统计学意义（ P ＜0．01）；β-catenin mRNA在舌癌和正常黏膜中均有表达，但表达量差异有统计学意义（ P ＜0．05）。低分化舌癌中Cox-2 mRNA、β-catenin mRNA及Survivin mRNA的表达量高于高中分化舌癌中的表达量（ P ＜0．05）。 Cox-2、β-catenin及Survivin在舌癌中的表达相互呈正相关（ P ＜0．01）。结论 Cox-2、β-catenin及Survivin在舌癌的发生发展过程中起重要作用，Cox-2、β-catenin及Survivin之间可能存在相互协同作用。
목적연구인설암조직중배양화매-2（ Cox-2）、β-련배단백（β-catenin ）급Survivin재기인수평적표체급상호관계，위설암적진단、치료급예후제공삼고의거。방법응용역전록-취합매련반응（ RT-PCR）방법검측Cox-2 mRNA、β-catenin mRNA급Survivin mRNA재60례설암화30례정상구강점막중적표체정황，분석타문재설암조직중표체적차이성화상관성。결과 Cox-2 mRNA화Survivin mRNA재설암조직중정강양성표체，이재정상구강점막균위음성표체，차이유통계학의의（ P ＜0．01）；β-catenin mRNA재설암화정상점막중균유표체，단표체량차이유통계학의의（ P ＜0．05）。저분화설암중Cox-2 mRNA、β-catenin mRNA급Survivin mRNA적표체량고우고중분화설암중적표체량（ P ＜0．05）。 Cox-2、β-catenin급Survivin재설암중적표체상호정정상관（ P ＜0．01）。결론 Cox-2、β-catenin급Survivin재설암적발생발전과정중기중요작용，Cox-2、β-catenin급Survivin지간가능존재상호협동작용。
Objective To investigate the expressions of cyclooxygenase-2(Cox-2),β-catenin and Survivin in tongue carcinoma in order to provide important clinical evidence for early diagnosis , therapy and prognosis of tongue carcinoma .Methods The expressions of Cox-2 mRNA,β-catenin mRNA and Survivin mRNA were detected by RT-PCR in 60 cases of primary tongue carcinoma and 30 cases of normal oral mucosa , and the difference of expressions and correlation among them were analyzed .Results The expressions Cox-2 mRNA and Survivin mRNA were strongly positive in tongue cancer ,however ,which were negative in normal oral mucosa ( P <0.01 );there was a significant difference in β-catenin expression quantity between tongue cancer and normal oral mucosa ( P <0.05).The expression quantity of Cox-2 mRNA,β-catenin mRNA and Survivin mRNA in poorly differentiated tongue cancer was significantly higher than that in well-differentiated and moderately-differentiated tongue cancer ( P <0.05 ).There was a positive correlation in their expressions in tongue cancer among Cox -2,β-catenin and Survivin ( P <0.01).Conclusion Cox-2,β-catenin and Survivin play an important role in the pathogenesis and development of tongue cancer , and there maybe a synergism among Cox-2,β-catenin and Survivin .