CAJ | 학술논문

目的制备尿清蛋白室内质控品，并进行室间质评调查。方法选取患者尿液样本，离心取上清液，加入乙二醇作为稳定剂，叠氮钠作为防腐剂，分装保存，观察稳定性和瓶间差，并进行室间质评调查。结果自制的尿清蛋白质控品除4℃保存的低值质控品外，CV均小于10％，有较好的稳定性，尤其在前3个月；此外，自制质控品的瓶间差较小，高值质控品瓶间差仅0．67％和0．81％，同时质控品的保存温度对瓶间差的影响很小。在全市的室间质评调查中低值质控品 CV 为63．3％（29．1％～70．3％）；高值质控品 CV 为20．5％（15．9％～22．3％）。结论研制的尿清蛋白质控品的稳定性较好，易于制备，可应用于临床实验室的室内质控和室间质评。
목적제비뇨청단백실내질공품，병진행실간질평조사。방법선취환자뇨액양본，리심취상청액，가입을이순작위은정제，첩담납작위방부제，분장보존，관찰은정성화병간차，병진행실간질평조사。결과자제적뇨청단백질공품제4℃보존적저치질공품외，CV균소우10％，유교호적은정성，우기재전3개월；차외，자제질공품적병간차교소，고치질공품병간차부0．67％화0．81％，동시질공품적보존온도대병간차적영향흔소。재전시적실간질평조사중저치질공품 CV 위63．3％（29．1％～70．3％）；고치질공품 CV 위20．5％（15．9％～22．3％）。결론연제적뇨청단백질공품적은정성교호，역우제비，가응용우림상실험실적실내질공화실간질평。
Objective To prepare internal quality control(QC) materials of urinary albumin and to investigate the external quality assessment .Methods Urine samples from patients were centrifuged to obtain supernatant ,and QC materials were prepared by adding ethylene glycol as stabilizer and sodium azide as preservative .QC materials were packaged and preserved ,and stability and between-bottles difference were analyzed .Then external quality as-sessment was performed among clinical laboratories .Results All QC materials ,except low-value QC materials pre-served at 4 ℃ ,were with low CV value(less than 10% ) and with fine stability .The between-bottles difference was small and not obviously influenced by preservation temperature ,and the differences between high-value QC materials were 0 .67% and 0 .81% .External quality assessment indicated that the CV values of low-and high-value QC materi-als were 63 .3% (29 .1% -70 .3% ) and 20 .5% (15 .9% -22 .3% ) .Conclusion The stability of self-prepared QC ma-terials of urinary albumin could be fine ,which could be utilized for internal QC and external quality assessment .