中外健康文摘
中外健康文摘
중외건강문적
WORLD HEALTH DIGEST
2013年
23期
22-24
,共3页
三唑仑%反相高效液相色谱法%血药浓度
三唑崙%反相高效液相色譜法%血藥濃度
삼서륜%반상고효액상색보법%혈약농도
Triazolam%HPLC%Plasma concentration
目的建立以反相高效液相色谱法测定人血浆中血药浓度三唑仑的方法。方法采用Diamonsil C18(250nm×4.6nm，5μm)色谱柱，流动相为甲醇：水(60：40)，流速为1.0ml/min，检测波长为234nm，柱温为25℃，采用蛋白沉淀法处理血浆样品。结果三唑仑血浆浓度在7.70～38.50μg(r=0.9997)范围内线形关系良好，低、中、高三种浓度的平均相对回收率分别为101.23％、103.88％，102.25％，RSD均小于3％。结论本方法简便、快速、准确、灵敏度高，可用于三唑仑血药浓度测定和人体药动学研究。
目的建立以反相高效液相色譜法測定人血漿中血藥濃度三唑崙的方法。方法採用Diamonsil C18(250nm×4.6nm，5μm)色譜柱，流動相為甲醇：水(60：40)，流速為1.0ml/min，檢測波長為234nm，柱溫為25℃，採用蛋白沉澱法處理血漿樣品。結果三唑崙血漿濃度在7.70～38.50μg(r=0.9997)範圍內線形關繫良好，低、中、高三種濃度的平均相對迴收率分彆為101.23％、103.88％，102.25％，RSD均小于3％。結論本方法簡便、快速、準確、靈敏度高，可用于三唑崙血藥濃度測定和人體藥動學研究。
목적건립이반상고효액상색보법측정인혈장중혈약농도삼서륜적방법。방법채용Diamonsil C18(250nm×4.6nm，5μm)색보주，류동상위갑순：수(60：40)，류속위1.0ml/min，검측파장위234nm，주온위25℃，채용단백침정법처리혈장양품。결과삼서륜혈장농도재7.70～38.50μg(r=0.9997)범위내선형관계량호，저、중、고삼충농도적평균상대회수솔분별위101.23％、103.88％，102.25％，RSD균소우3％。결론본방법간편、쾌속、준학、령민도고，가용우삼서륜혈약농도측정화인체약동학연구。
Objective:To establish a Rp-HPLC method for the determination of triazolam in human plasma.Method:The HPLc separation was achieved on a Diamonsil C18 column(250mm×4.6 mm,5μm),and the mobile phase consisted of MeOH-H20(60:40).The detection wavelength wan 254 nm.flow rate was 1.0ml/L.and column temperature was25℃. The protein precipitation method was used to treat the plasma samples.Result:The linear range of diazepam was7.70~38.50μg (r=0.9997),the average relative recovery of low,middle and high concentration was101.23%,103.88%and 102.25%, respectively,and the RSDs were less than 3%.Conclusion:The method is simple,rapid,accurate and sensitive for the plasma concentration monitoring and clinical pharmacokinetic studies of triazolam.
