CAJ | 학술논문

目的分析不同乙肝检测模式中Pre-S1抗原与HBV-DNA的关系。方法采用酶联免疫吸附试验（ELISA），测定Pre-S1和HBcAg。用荧光定量PCR法检测HBV-DNA。比较Pre-S1抗原在各种模式中的阳性率及Pre-S1抗原与HBV-DNA的关系。结果 HBsAg （+）、HBeAb（+）、HBcAb（+）模式中，Pre-S1抗原（+）阳性率为86%，HBsAg（+）、HBeAb（+）、HBcAb（+）模式中，Pre-S1抗原（+）阳性率为40%，HBsAg（+）、HBcAb（+）模式中，Pre-S1抗原（+）阳性率为25%，结果具有统计学意义（P＜0.05）。各组模式中， Pre-S1抗原阳性率与HBV-DNA比较，无显著性差异（P＞0.05）。结论 Pre-S1抗原可以作为判断乙肝的补充指标，对临床实践有指导意义。
목적분석불동을간검측모식중Pre-S1항원여HBV-DNA적관계。방법채용매련면역흡부시험（ELISA），측정Pre-S1화HBcAg。용형광정량PCR법검측HBV-DNA。비교Pre-S1항원재각충모식중적양성솔급Pre-S1항원여HBV-DNA적관계。결과 HBsAg （+）、HBeAb（+）、HBcAb（+）모식중，Pre-S1항원（+）양성솔위86%，HBsAg（+）、HBeAb（+）、HBcAb（+）모식중，Pre-S1항원（+）양성솔위40%，HBsAg（+）、HBcAb（+）모식중，Pre-S1항원（+）양성솔위25%，결과구유통계학의의（P＜0.05）。각조모식중， Pre-S1항원양성솔여HBV-DNA비교，무현저성차이（P＞0.05）。결론 Pre-S1항원가이작위판단을간적보충지표，대림상실천유지도의의。
Objective To analyse the different hepatitis b detection pattern in the Pre-S1 antigen and HBV-DNA . Method Used the enzyme-linked immunosorbent assay (ELISA),and determined the Pre-S1 and HBcAg. Used lfuorescence quantitative PCR method to detect HBV-DNA. Compared the Pr-S1 antigen positive rate in all kinds of patterns and the Pre-S1 antigen and HBV-DNA. Result In HBsAg(+), HBeAb(+)and HBcAb(+) mode, the Pre-S1 antigen (+) positive rate was 86%, the HBsAg(+), HBeAb(+)and HBcAb(+)mode, the Pre-S1 antigen (+) positive rate was 40%, the HBsAg(+)and HBcAb(+)mode, the Pre-S1 antigen (+) positive rate was 25%, the results had statistical signiifcance(P<0.05). Conclusion The Pre-S1 antigen can be a supplementary index, in clinical practice it can judge hepatitis b as a guidance.