CAJ | 학술논문

目的探讨SIRT1在扩张型心肌病（DCM）大鼠心肌中的表达及其意义。方法采用腹腔注射阿霉素8周建立大鼠DCM模型，彩色多普勒检测大鼠左心室结构和功能，采用免疫组织化学方法及Western blot技术检测心肌组织中SIRT1蛋白表达水平。结果与正常组相比，DCM组大鼠左室舒张期末径和左室收缩期末径均明显增大，左室射血分数明显下降；DCM大鼠心肌组织中SIRT1蛋白表达水平明显下调。结论 SIRT1在DCM心肌中表达下调，提示其与DCM的发生与发展相关。
목적탐토SIRT1재확장형심기병（DCM）대서심기중적표체급기의의。방법채용복강주사아매소8주건립대서DCM모형，채색다보륵검측대서좌심실결구화공능，채용면역조직화학방법급Western blot기술검측심기조직중SIRT1단백표체수평。결과여정상조상비，DCM조대서좌실서장기말경화좌실수축기말경균명현증대，좌실사혈분수명현하강；DCM대서심기조직중SIRT1단백표체수평명현하조。결론 SIRT1재DCM심기중표체하조，제시기여DCM적발생여발전상관。
Objective To demenstrate the relationship of SIRT1 expression and heart dysfunction from dilated cardiomyopathy (DCM) rat.Methods The rat model of DCM was established by intraperitoneal injection of adriamycin once a week for 8 weeks. Left ventricular end diastolic diameter,1eft ventricular end systolic diameter and left ventricular ejection fraction were measured by Doppler. The expression of SIRT1 was detected with immunohistochemistry and Western Blotting,respectively.Results Compared with the normal rats,both the LVDD and LVDS were increased and the LVEF decreased.The expression of SIRT1 was down-regulated significantly in the rats with DCM.Conclusion The expression of SIRT1 downregulation in the cardiomyocytes may be associated with the progression and pathogenesis of DCM.