中国保健营养(上旬刊)
中國保健營養(上旬刊)
중국보건영양(상순간)
China Health Care & Nutrition
2014年
4期
1801-1803
,共3页
高效液相色谱法%紫外分光光度法%尼群地平片%含量测定
高效液相色譜法%紫外分光光度法%尼群地平片%含量測定
고효액상색보법%자외분광광도법%니군지평편%함량측정
HPLC UV spectrophotometry%Nitrendipine Tablet Determination
目的:本文研究并比较了高效液相色谱法和紫外分光光度法测定尼群地平片含量的方法。方法高效液相色谱法,C18(4.6×150nm 5um)色谱柱,甲醇-水(65:35)为流动相。流速:1.0ml/min,检测波长为237nm,进样量为20ul;紫外分光光度计,测定波长为237nm。结果高效液相色谱法:尼群地平片在0.02000-0.1000mg/ml浓度范围内有良好的线性关系r=0.9999,平均回收率为100.1%(RSD=0.13%);紫外分光光度法:尼群地平片在2.00-10.00ug/ml范围内与吸收度呈线性关系(r=0.9993),平均回收率为102.5%(RSD=1.71%)。结论高效液相色谱是一种简便、快速、精确、准确的分析方法,有一定的实用意义。
目的:本文研究併比較瞭高效液相色譜法和紫外分光光度法測定尼群地平片含量的方法。方法高效液相色譜法,C18(4.6×150nm 5um)色譜柱,甲醇-水(65:35)為流動相。流速:1.0ml/min,檢測波長為237nm,進樣量為20ul;紫外分光光度計,測定波長為237nm。結果高效液相色譜法:尼群地平片在0.02000-0.1000mg/ml濃度範圍內有良好的線性關繫r=0.9999,平均迴收率為100.1%(RSD=0.13%);紫外分光光度法:尼群地平片在2.00-10.00ug/ml範圍內與吸收度呈線性關繫(r=0.9993),平均迴收率為102.5%(RSD=1.71%)。結論高效液相色譜是一種簡便、快速、精確、準確的分析方法,有一定的實用意義。
목적:본문연구병비교료고효액상색보법화자외분광광도법측정니군지평편함량적방법。방법고효액상색보법,C18(4.6×150nm 5um)색보주,갑순-수(65:35)위류동상。류속:1.0ml/min,검측파장위237nm,진양량위20ul;자외분광광도계,측정파장위237nm。결과고효액상색보법:니군지평편재0.02000-0.1000mg/ml농도범위내유량호적선성관계r=0.9999,평균회수솔위100.1%(RSD=0.13%);자외분광광도법:니군지평편재2.00-10.00ug/ml범위내여흡수도정선성관계(r=0.9993),평균회수솔위102.5%(RSD=1.71%)。결론고효액상색보시일충간편、쾌속、정학、준학적분석방법,유일정적실용의의。
Objce tive To establish method and comparision for HPLC and UV Determination of nitrendipine Tablet .Methods Used high-performance liquid chro-matography,Used as C18(4.6 ×150nm 5um)chromatographic column,Methanol-water(volume ratio of 65:35)as mobile phase.Flow rate:1.0 ml /min,Detection at 237 nm,sample volume for the 20 ul;by ultraviolet spectrophotometer,for the wavelength of 237 nm.Results HPLC:Nitrendipine Tablet has perfect linear correlation in the range of 0.02000-0.1000mg/ml,r=0.9999,The average recovery rate is 100.1%,and RSD=0.13%;uv:Nitrendipine Tablet has linear correlation in the range of 2.00-10.00ug/ml,r=0.9993,The average recovery rate is 102.5%,and RSD=1.71%;Conclusion The method is simple,convenient,and accurate Nitrendipine Tablet in the concentration of a good linear relationship,with an average recovery rate of100%.It can be used to control the content of Nitrendipine Tablet.