CAJ | 학술논문

目的：掌握目前南京市艾滋病筛查实验室HIV抗体检测工作质量、实验室管理水平，发现各实验室检测过程中可能存在的问题，促进其整体检测能力提高。方法：以卫生部《艾滋病检测工作技术规范》《南京地区艾滋病检测工作管理规范》为主要依据，通过发放质控品进行盲样考核和专家实验室现场督导相结合的方式对全市73家艾滋病筛查实验室进行考评。其中盲样考核包括检测结果的准确性以及实验的规范性，两部分各占25%；实验室现场督导占50%；结果总分100分。结果：73家艾滋病筛查实验室酶联法（ELISA）试验结果符合率均为100%，平均得分25分；实验的规范性平均得分为22.4分；实验室现场督导平均得分为49.3分。结论：南京艾滋病筛查实验室检测能力均较好，能够满足艾滋病筛查工作的需要。但是在实验过程中的规范化管理及实验室质量管理两方面均需进一步提高和完善。
목적：장악목전남경시애자병사사실험실HIV항체검측공작질량、실험실관리수평，발현각실험실검측과정중가능존재적문제，촉진기정체검측능력제고。방법：이위생부《애자병검측공작기술규범》《남경지구애자병검측공작관리규범》위주요의거，통과발방질공품진행맹양고핵화전가실험실현장독도상결합적방식대전시73가애자병사사실험실진행고평。기중맹양고핵포괄검측결과적준학성이급실험적규범성，량부분각점25%；실험실현장독도점50%；결과총분100분。결과：73가애자병사사실험실매련법（ELISA）시험결과부합솔균위100%，평균득분25분；실험적규범성평균득분위22.4분；실험실현장독도평균득분위49.3분。결론：남경애자병사사실험실검측능력균교호，능구만족애자병사사공작적수요。단시재실험과정중적규범화관리급실험실질량관리량방면균수진일보제고화완선。
Objective:To investigate the quality of HIV antibody testing in Nanjing AIDS screening laboratory and the level of laboratory management,find out the possible existence of the problems in the process of laboratory testing,upgrade the quality of HIV testing in Nanjing.Method:With the ministry of health AIDS test specification,the Nanjing area code for HIV testing work management as the main basis,73 screening laboratories in Nanjing were examined and evaluated.Samples for quality control check were extended to all laboratories and experts on-site laboratory examination as well.The assessment of unknown sample concluded the accuracy of the test results and the standardization of the experiment.Two parts each accounted for 25%,laboratory site supervision accounted for 50%;results total of 100 points.Result:The coincidence rate of ELISA test of 73 laboratories was all 100% respectively.The score of standardize management of them was 22.4 points.The score of on-site laboratory examination of them was 49.3 points. Conclusion:The test ability of 73 laboratories is good,but the standardize management and the laboratory quality management should be enhanced and perfected.