中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2013年
29期
6-7
,共2页
张国荣%台桂香%张淑芳%池明%方军
張國榮%檯桂香%張淑芳%池明%方軍
장국영%태계향%장숙방%지명%방군
构建%生物学检测%体外研究%组织工程肌腱
構建%生物學檢測%體外研究%組織工程肌腱
구건%생물학검측%체외연구%조직공정기건
Construction%Biological assay%In-vitro study%Tissue-engineered tendon
目的:构建应用于生物学研究的组织工程肌腱,为体外研究中检测胶原纤维的形态结构提供可行的方法手段。方法:培养大鼠肌腱细胞,构建无支架的组织工程肌腱,并检测其光镜结构和电镜结构。结果:构建的组织工程肌腱表面光滑,结构紧密,细胞生存良好,细胞外基质分泌丰富。具有波浪形的胶原纤维,胶原纤维排列规则,直径大小相对均一,但小于正常肌腱纤维直径。结论:本研究构建的组织工程肌腱不含支架材料,结构与损伤肌腱类似,可以检测多种生物学指标,适于肌腱损伤修复的体外研究。
目的:構建應用于生物學研究的組織工程肌腱,為體外研究中檢測膠原纖維的形態結構提供可行的方法手段。方法:培養大鼠肌腱細胞,構建無支架的組織工程肌腱,併檢測其光鏡結構和電鏡結構。結果:構建的組織工程肌腱錶麵光滑,結構緊密,細胞生存良好,細胞外基質分泌豐富。具有波浪形的膠原纖維,膠原纖維排列規則,直徑大小相對均一,但小于正常肌腱纖維直徑。結論:本研究構建的組織工程肌腱不含支架材料,結構與損傷肌腱類似,可以檢測多種生物學指標,適于肌腱損傷脩複的體外研究。
목적:구건응용우생물학연구적조직공정기건,위체외연구중검측효원섬유적형태결구제공가행적방법수단。방법:배양대서기건세포,구건무지가적조직공정기건,병검측기광경결구화전경결구。결과:구건적조직공정기건표면광활,결구긴밀,세포생존량호,세포외기질분비봉부。구유파랑형적효원섬유,효원섬유배렬규칙,직경대소상대균일,단소우정상기건섬유직경。결론:본연구구건적조직공정기건불함지가재료,결구여손상기건유사,가이검측다충생물학지표,괄우기건손상수복적체외연구。
Objective:To Construct tissue-engineered tendon for biological assay to provide practical methods and means for detecting the morphology of collagen fiber in in-vitro study.Method:Rat tendon cells were cultured and were used to construct scaffold-free tissue-engineered tendon. The structure was detected by light microscope and electron microscope.Result:The tissue engineered-tendon with living cells and collagen matrix has a smooth surface and compact structure. It has bundles of highly crimped fibers and a more uniform distribution of smaller diameter collagen fibrils than native tissue.Conclusion:This tissue-engineered tendon is scaffold-free and has a similar structure with injured tendon. It can be used for many biological assays and is applicable for studying tendon injury in vitro.