CAJ | 학술논문

目的：探讨红花注射液对肝癌HepG-2细胞增殖的影响及分子机制。方法应用红花注射液（0、50、150、250 g/L）处理HepG-2细胞，经AnnexinⅤ-FITC/PI双染荧光显微镜观察细胞凋亡的形态学改变，实时荧光定量PCR检测AKT mRNA表达，蛋白质印迹（ Western blot ）检测AKT、p-AKT蛋白表达。结果50、150、250 g/L的红花注射液作用于HepG-2细胞48 h后，荧光显微镜下呈现典型的凋亡细胞形态；细胞内AKT mRNA表达水平显著降低，AKT、p-AKT蛋白表达水平也显著下调，且变化趋势均呈一定的剂量依赖关系。结论红花注射液可能通过抑制AKT信号通路，阻碍肝癌HepG-2细胞的增殖及诱导凋亡。
목적：탐토홍화주사액대간암HepG-2세포증식적영향급분자궤제。방법응용홍화주사액（0、50、150、250 g/L）처리HepG-2세포，경AnnexinⅤ-FITC/PI쌍염형광현미경관찰세포조망적형태학개변，실시형광정량PCR검측AKT mRNA표체，단백질인적（ Western blot ）검측AKT、p-AKT단백표체。결과50、150、250 g/L적홍화주사액작용우HepG-2세포48 h후，형광현미경하정현전형적조망세포형태；세포내AKT mRNA표체수평현저강저，AKT、p-AKT단백표체수평야현저하조，차변화추세균정일정적제량의뢰관계。결론홍화주사액가능통과억제AKT신호통로，조애간암HepG-2세포적증식급유도조망。
Objective To investigate the effect and mechanism of Safflower injection on cell proliferation of human hepatoma cells HepG-2 in vitro.Methods HepG-2 cells were treated by different concentrations (0, 50, 150, 250 g/L) of safflower injection .Fluorescence microscopy was applied to test the morphological changes of apoptosis by AnnexinⅤ-FITC/PI staining.Quantitative real-time PCR was carried to assess the mRNA expression of AKT.Western blot were used to observe the protein expression levels of AKT and p -AKT in HepG-2 cells.Results After treated with Safflower injection , HepG-2 cells presented the typical modality of early apoptosis; with significantly dose -dependent down-regulation in AKT mRNA, and AKT and p-AKT proteins.Conclusion Safflower injection inhibits cell prolifera-tion and induces apoptosis in HepG -2 cells through AKT signaling pathway .