CAJ | 학술논문

目的探讨简易效佳的菌种保存方法，以便临床、教学和科研工作之用。方法用中药芡实、山药分别加入营养肉汤中作为菌种保存液，对23种细菌进行滤纸冰冻法保存。结果保存2年后取出培养，各菌存活率达100%。结论芡实或山药肉汤作为菌种保存液，用滤纸冰冻法可较长时间保存菌种。
목적탐토간역효가적균충보존방법，이편림상、교학화과연공작지용。방법용중약검실、산약분별가입영양육탕중작위균충보존액，대23충세균진행려지빙동법보존。결과보존2년후취출배양，각균존활솔체100%。결론검실혹산약육탕작위균충보존액，용려지빙동법가교장시간보존균충。
To investigate the effect simple bacteria to preserve the best method to clinical, teaching and research activities. Methods: Euryale and Chinese yam were added into the nutrient broth as a preservation solution species, 23 kinds of bacteria on the filter paper method to preserve frozen. Results: Save the results after 2 years out of training, the survival rate of the bacteria was 100%. Conclusion : Euryale or yam broth preservation solution as a strain with filter paper method can be frozen to preserve bacteria a longer period of time.