山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2014年
19期
26-28
,共3页
妊娠并发症%子痫前期%动物模型%内毒素
妊娠併髮癥%子癇前期%動物模型%內毒素
임신병발증%자간전기%동물모형%내독소
medical Complications in pregnancy%preeclampsia%rat model%lipopolysaccharides
目的：用低剂量内毒素诱导构建子痫前期大鼠模型。方法将12只妊娠大鼠随机分为观察组6只和对照组6只，于妊娠第14天，观察组尾静脉缓慢注射2 mL 内毒素（1 mg/kg），对照组注射2 mL生理盐水。测量两组大鼠妊娠第7、13、15、17、19天时的血压，采用免疫比浊法测定两组大鼠妊娠第12、15天的24 h尿蛋白。妊娠第20天大鼠均未分娩，对其进行剖宫取胎，观察仔鼠情况，并取胎盘组织做HE染色，观察病理形态学改变。结果妊娠第15、17、19天时观察组大鼠收缩压均明显升高（高于基础值30 mmHg），对照组血压无明显变化，两组比较，P均＜0．05。妊娠第15天，观察组24 h尿蛋白（高于基础值2倍）为（6．07±0．75）mg/24 h，对照组为（1．90±0．43） mg/24 h，两组比较，P＜0．05。观察组组织病理学检查可见基带炎性细胞浸润、迷路带组织核碎裂、纤维蛋白沉积等胎盘炎性改变。结论低剂量内毒素能诱导大鼠产生高血压、蛋白尿、胎盘炎性改变等子痫前期表现，子痫前期大鼠模型构建成功。
目的：用低劑量內毒素誘導構建子癇前期大鼠模型。方法將12隻妊娠大鼠隨機分為觀察組6隻和對照組6隻，于妊娠第14天，觀察組尾靜脈緩慢註射2 mL 內毒素（1 mg/kg），對照組註射2 mL生理鹽水。測量兩組大鼠妊娠第7、13、15、17、19天時的血壓，採用免疫比濁法測定兩組大鼠妊娠第12、15天的24 h尿蛋白。妊娠第20天大鼠均未分娩，對其進行剖宮取胎，觀察仔鼠情況，併取胎盤組織做HE染色，觀察病理形態學改變。結果妊娠第15、17、19天時觀察組大鼠收縮壓均明顯升高（高于基礎值30 mmHg），對照組血壓無明顯變化，兩組比較，P均＜0．05。妊娠第15天，觀察組24 h尿蛋白（高于基礎值2倍）為（6．07±0．75）mg/24 h，對照組為（1．90±0．43） mg/24 h，兩組比較，P＜0．05。觀察組組織病理學檢查可見基帶炎性細胞浸潤、迷路帶組織覈碎裂、纖維蛋白沉積等胎盤炎性改變。結論低劑量內毒素能誘導大鼠產生高血壓、蛋白尿、胎盤炎性改變等子癇前期錶現，子癇前期大鼠模型構建成功。
목적：용저제량내독소유도구건자간전기대서모형。방법장12지임신대서수궤분위관찰조6지화대조조6지，우임신제14천，관찰조미정맥완만주사2 mL 내독소（1 mg/kg），대조조주사2 mL생리염수。측량량조대서임신제7、13、15、17、19천시적혈압，채용면역비탁법측정량조대서임신제12、15천적24 h뇨단백。임신제20천대서균미분면，대기진행부궁취태，관찰자서정황，병취태반조직주HE염색，관찰병리형태학개변。결과임신제15、17、19천시관찰조대서수축압균명현승고（고우기출치30 mmHg），대조조혈압무명현변화，량조비교，P균＜0．05。임신제15천，관찰조24 h뇨단백（고우기출치2배）위（6．07±0．75）mg/24 h，대조조위（1．90±0．43） mg/24 h，량조비교，P＜0．05。관찰조조직병이학검사가견기대염성세포침윤、미로대조직핵쇄렬、섬유단백침적등태반염성개변。결론저제량내독소능유도대서산생고혈압、단백뇨、태반염성개변등자간전기표현，자간전기대서모형구건성공。
Objective To induce the rats to copy the rat model with preeclampsia by using low-dose lipopolysacchar-ides.Methods:twelve pregnant rats were randomly divided into 2 groups: experimental group and normal control group , with 6 rats in each group.The experimental group were injected with 2ml of lipopolysaccharides solution of 1μg/kg through tail veins on day 14 of pregnancy, while the control group were injected with 2ml saline.The blood pressure on day 7,13, 15,17,19 were monitored, and the 24-hour proteinuria on day 12,15 were measured by Immune turbidimetry .All the rats had no delivery on day 20, and then the uterine cesarean delivery were made to them , and observed the status of the new-born mouse, and got the placentas to make HE staining and observed the pathological changes .Results:The systolic pres-sure of the experimental group rose significantly on day 15,17 and 19 of pregnancy, which was higher more than 30 mmHg of the basic pressure .The blood pressure of the normal control group had no obvious change .The P of both two groups were less than 0.05 while they compared with each other .On day 15 of pregnancy, the 24-hour proteinuria(more than twice as much as the basic pressure) of the experimental group was(6.07 ±0.75)mg/24h, while that of the normal group was (1. 90 ±0.43)mg/24h, and P<0.05 when these 2 groups compared with each other .The experimental group showed placen-tal inflammatory changes such as baseband inflammatory cell infiltration , labyrinth zone tissue nuclear fragmentation and fi-brin deposition by examination of the pathology .Conclusion:low-dose lipopolysaccharides could induce preeclampsia per-formances such as hypertension , proteinuria and placental inflammatory changes in rats .The The rat model with preeclamp-sia was successfully established .
