CAJ | 학술논문

目的：研究炎症对声带间充质干细胞（vocal fold mesenchymal stem cells，VF-MSCs）生物学行为的影响，为炎症声带损伤修复提供一定的理论依据。方法正常和慢性炎症声带组织分别取自于下咽癌喉全切除的正常声带组织及水肿型声带息肉手术患者切除的标本，所取组织大小约1mm×1mm，每种组织3例，对其行HE、Masson三色染色和Elastin Van Gieson（EVG）染色，观察炎性声带组织较正常组织胶原纤维和弹性纤维改变情况；通过细胞克隆形成实验和MTT细胞生长曲线实验研究炎症对VF-MSCs增殖能力的影响；通过VF-MSCs定向向成骨和成脂细胞分化，研究炎症对VF-MSCs多向分化能力的影响。结果Masson三色和EVG染色结果显示炎性声带组织固有层细胞外基质（extracellular matrix，ECM）胶原纤维变粗，含量增多，弹力纤维变细，含量减少；细胞克隆形成实验和MTT细胞生长曲线结果显示炎性声带间充质干细胞（inflammatory vocal foldmexen-chymalstemcells，I-VF-MSCs）较正常声带间充质干细胞（normal vocal fold mesenchymal stem cells，N-VF-MSCs）增殖能力增强；细胞成骨和成脂分化实验结果显示I-VF-MSCs较N-VF-MSCs多向分化能力减弱。结论炎性声带组织较正常声带组织弹性减弱，抗压力增强，炎症促进VF-MSCs增殖能力，抑制VF-MSCs多向分化能力。
목적：연구염증대성대간충질간세포（vocal fold mesenchymal stem cells，VF-MSCs）생물학행위적영향，위염증성대손상수복제공일정적이론의거。방법정상화만성염증성대조직분별취자우하인암후전절제적정상성대조직급수종형성대식육수술환자절제적표본，소취조직대소약1mm×1mm，매충조직3례，대기행HE、Masson삼색염색화Elastin Van Gieson（EVG）염색，관찰염성성대조직교정상조직효원섬유화탄성섬유개변정황；통과세포극륭형성실험화MTT세포생장곡선실험연구염증대VF-MSCs증식능력적영향；통과VF-MSCs정향향성골화성지세포분화，연구염증대VF-MSCs다향분화능력적영향。결과Masson삼색화EVG염색결과현시염성성대조직고유층세포외기질（extracellular matrix，ECM）효원섬유변조，함량증다，탄력섬유변세，함량감소；세포극륭형성실험화MTT세포생장곡선결과현시염성성대간충질간세포（inflammatory vocal foldmexen-chymalstemcells，I-VF-MSCs）교정상성대간충질간세포（normal vocal fold mesenchymal stem cells，N-VF-MSCs）증식능력증강；세포성골화성지분화실험결과현시I-VF-MSCs교N-VF-MSCs다향분화능력감약。결론염성성대조직교정상성대조직탄성감약，항압력증강，염증촉진VF-MSCs증식능력，억제VF-MSCs다향분화능력。
Objective The aim of the study was to investigate the effects of inflammation on the biological be-haviors of the VF -MSCs and provide the theoretic basis for the repair of the vocal folds which were damaged by in-flammation .Methods The inflammatory vocal fold tissues and normal vocal fold tissues were respectively derived from the vocal cord polyp and normal tissues of the hypopharyngeal carcinoma patients .The HE staining ,masson trichrome staining and elastin van gieson (EVG) staining were performed to detect the effects of inflammation on the collagenous fiber and elastic fibers of the vocal fold lamina propria .The cell colony formation analysis and MTT cell growth curve were used to detect the effects of inflammation on the proliferation of VF -MSCs .The effects of in-flammation on the multi-directional differentiation of VF -MSCs were evaluated by inducing the VF -MSCs to dif-ferentiate into osteoblasts and lipoblasts .Results The results of masson trichrome staining and EVG staining showed that in the inflammatory vocal fold lamina propria collagen fibers became thicker and the amount of collagen fibers increased ,while elastic fibers became thinner and the amount of elastic fibers decreased .Compared with the vocal fold mesenchymal stem cell (VF-MSCs) in normal vocal folds ,VF-MSCs in inflammatory vocal folds pro-liferated more significantly ,but the osteogenic differentiation and adipogenic differentiation of VF -MSCs in inflammatory vocal folds were restrained .Conclusion Inflammation enhanced the compressive resistance ,abated the elasticity , and restrained the multi -directional differentiation of VF -MSCs .